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1、-北京市心肺血管疾病研究所硕士学位论文Bloodpressureisregulatedbymanyfactors,includingfunctionalfactors,eg.increasedsmoothmusculartension;structuralfactors,eg.cellproliferation;modulatoryfactors,eg.nerve,hormoneandsignal-transconductionpassway,etc,.Thosefactorslosetheirbalancegrad
2、ually,andcontinuouslyleadtohypertensionintheend.ThestudyillustratedthattheexpressionofHSGwaslowerinthehypertensiveanimal,butnormalinthenormaltensiveanimal.Asaresult,wecanspeculatethatthisgeneisoneofthemainreasonsofhypertrophyofVSMCs.Therefore,whatdifferencesexi
3、stincoronaryheartdiseasespatientswithandwithouthypertensionincellularandmolecularlevel?Toanswerthisquestion,wesetoutourresearch,andexpectedtofindsomeevidencetoprovidereferenceforhypertensiondiagnosis,treatmentandprevention.[Methods]Accordingtodiagnosticcriterio
4、nofessentialhypertension(hypertensionpreventiveandtherapeuticguideline,China,2005),54coronaryheartdiseasepatients,whowereperformedcoronaryarterybypassgraft(CABG)operation,wereenrolledin.Theyweredividedintohypertensivegroup(n=28)andnormotensivegroup(n=26).Thepre
5、operationvenousbloodsamplesweretakenfrombothgroupsandtestedserumbiochemicalitemsbybiochemicalanalyzingmachines(DimensionRL,American),andvasoactivepeptides(AngiotensinI,AngiotensinII,plasmareninactivity,aldosterone)oftheReninAngiotensinAldosteronesystemaswellase
6、ndothelin(ET)byradioimmunoassay.Duringtheoperation,theredundantbridge-vessels(saphenousvein)about2centimeterswerecollected.Someofthemwereusedforvascularsmoothmusclecells(VSMCs)culture;theotherswerefrozenandstoredinliquidnitrogen.TotalRNAwasextractedfromWBC,brig
7、de-vesselsandcultureVSMCsusing7---北京市心肺血管疾病研究所硕士学位论文TRIZOLaccordingtothemanufacturer’sdirections,andfinishedreversetranscription(RT)reactionusingkits(Promega).PrimersforthegeneencodingthehumanhyperplasiasuppressorgeneweredesignedspecificallybyPremierPrimer5.0so
8、ftwareandpreparedbyShanghaiGenecoreBiotechnologiesCompany.Polymerasechainreaction(PCR)wasperformedonthepropercondition.Reactionproductsthenwerepurifiedandsentforsequencedete
