资源描述:
《expression of transforming growth factor》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、Expressionoftransforminggrowthfactor:DanCao,LinLiu,ETHODS:28healthyadultSprague-Dalydividedintotalcontrol(CON)anddiabetesmellitus(DM).Diabetesefluorescencequantitativereversetranscriptionpolymerasechainreaction(QRT-PCR).RESULTS:ThemRNAlevelofTβR1andTβR2RNAlevelofTβR1an
2、dTβR2ayplayimportantroleinthepathogenesisofdiabeticretinopathy.【关键词】diabeticretinopathyINTRODUCTIONDiabeticretinopathy(DR)isthemainplicationofdiabetesmellitus(DM),andisoneofthemostleadingcausesofblindnessicroangiopathycharacterizedbysmallvesseldamageandocclusion.Theear
3、liestpathologicchangesarethickeningofthecapillaryendothelialbasementmembraneandreductionofpericytes.ATERIALSANDMETHODSExperimentalAnimals28adultmaleSprague-DaalCenter.Adaptabilityfeedingfor2entmoniaconcentrationof≤20ppm,arelativehumidityof40%-70%,24hnaturallightillumin
4、ation,andaroomtem-peratureof18to22℃.Feedp-likeratfeedstuff,lydividedintotalcontrol(CON)and16inthediabetesmellitus(DM).groupdied.Thebloodglucoselevelandbodyinedregularlyafterthat.Inexperimentperiod,15ratsaresuccessfulinducedDM,1ratdied.So7inDMentalanimalsinationoftheirb
5、odyovedandtheretinamediatelyplacedintoliquidnitrogenforpreservation.)ethy-lrhodamine(TAMRA)ersequenceofgeneTβR1,TβR2and18sofrats,asersynthesisisasfollo:FAM5'>CAGAGCTGTGAGGCCTTGAGAGTG<3'TAMRA;T(RII-F:5'>CCCTACTCTGTCTGTGGATGA<3';T(RII-R:5'>GACGTCATTTCCCAGA
6、GTAC<3';T(RII-TM:FAM5'>CAGGTGGGAACAGCGAGATACATGG<3'TAMRA;18s-F:5'>GTAACCCGTTGAACCCCATT<3';18s-R:5'>CCATCCAATCGGTAGTAGCG<3';18s-TM:FAM5'>ATGGGGATCGGGGATTGCAAT<3'TAMRA.QuantitativePCRReactionandAnalysisThequantitativePCRdeterminationandanalysis
7、edinaccordanceethodsintroducedinthereference[5].B.Physiologicalactionsandclinicalapplicationsoftransforminggroadsignaling.TrendsBiochemSci,2004;29:265-2733DerynckR,ZhangYE.Smad-dependentandsmad-independentpathilysignaling.Nature,2003;425:577-5844HiraseK,IkedaT,Sotozono
8、C,NishidaK,Sainggrool,1998;116:738-7415ManXB,TangL,QiuXH,TanYX,HingY,C,L,KapetaniosAD,DonatiG,RedardM,GabbianiG,Pourn