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1、Expressionoftransforminggrowthfactor：DanCao,LinLiu,ETHODS:28healthyadultSprague-Dalydividedintotalcontrol(CON)anddiabetesmellitus(DM).Diabetesefluorescencequantitativereversetranscriptionpolymerasechainreaction(QRT-PCR).RESULTS:ThemRNAlevelofTβR1andTβR2RNAlevelofTβR1andTβR2aypla
2、yimportantroleinthepathogenesisofdiabeticretinopathy.【关键词】diabeticretinopathyINTRODUCTIONDiabeticretinopathy(DR)isthemainplicationofdiabetesmellitus(DM),andisoneofthemostleadingcausesofblindnessicroangiopathycharacterizedbysmallvesseldamageandocclusion.Theearliestpathologicchang
3、esarethickeningofthecapillaryendothelialbasementmembraneandreductionofpericytes.ATERIALSANDMETHODSExperimentalAnimals28adultmaleSprague-DaalCenter.Adaptabilityfeedingfor2entmoniaconcentrationof≤20ppm,arelativehumidityof40%-70%,24hnaturallightillumination，andaroomtem-peratureof18
4、to22℃.Feedp-likeratfeedstuff,lydividedintotalcontrol(CON)and16inthediabetesmellitus(DM).groupdied.Thebloodglucoselevelandbodyinedregularlyafterthat.Inexperimentperiod,15ratsaresuccessfulinducedDM,1ratdied.So7inDMentalanimalsinationoftheirbodyovedandtheretinamediatelyplacedintoli
5、quidnitrogenforpreservation.)ethy-lrhodamine(TAMRA)ersequenceofgeneTβR1,TβR2and18sofrats,asersynthesisisasfollo:FAM5'>CAGAGCTGTGAGGCCTTGAGAGTG<3'TAMRA;T(RII-F:5'>CCCTACTCTGTCTGTGGATGA<3';T(RII-R:5'>GACGTCATTTCCCAGAGTAC<3';T(RII-TM:FAM5'>CAGGTGGGAACAGCGAGATAC
6、ATGG<3'TAMRA;18s-F:5'>GTAACCCGTTGAACCCCATT<3';18s-R:5'>CCATCCAATCGGTAGTAGCG<3';18s-TM：FAM5'>ATGGGGATCGGGGATTGCAAT<3'TAMRA.QuantitativePCRReactionandAnalysisThequantitativePCRdeterminationandanalysisedinaccordanceethodsintroducedinthereference[5].B.Physiologi
7、calactionsandclinicalapplicationsoftransforminggroadsignaling.TrendsBiochemSci,2004;29:265-2733DerynckR,ZhangYE.Smad-dependentandsmad-independentpathilysignaling.Nature,2003;425:577-5844HiraseK，IkedaT,SotozonoC,NishidaK,Sainggrool,1998;116:738-7415ManXB,TangL,QiuXH,TanYX,HingY,C
8、,L,KapetaniosAD,DonatiG,RedardM,GabbianiG,Pourn
