板栗多糖类物质提取方法优化与不同品种(系)含量测定

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1、ABSTRACTPolysaccharide，animportantactivematerial，iswidelyexistinplants，andhaveawideprospectofdevelopmentandapplicationduetoitsuniqueactivefunction．Basedontheexperimentalmaterialsforyanshanchestnut(CastaneamollissimaBlume)kernel，researchonthemeansofdifferentextractionofpol

2、ysaccharideinChinesechestnutkernel，tooptimizetheextractiontechnologyofchestnutpolysaccharide，andthentheoptimalconditionsforChinesechestnutkernelpolysaccharidewereobtainedbymeansofultrasound—AssistedExtraction．Theresultsareasfollows：1．Polysaccharideswereextractedfromchestn

3、utusinghotwaterextractionandmicrowave·-assistedextractionandhydrochloricacid··assistedextractionandultrasound—assistedextraction．Theoptimalconditionsofultrasonicextractionwere：extractiontemperature30℃，liquid—solidratio40：l(mL：g)，extractiontime50min，ultrasonicpower870Wthey

4、ieldofpolysaccharideswas26．25％．2．Tooptimizethedecolorizationtechnologyofpolysaccharideactivecarbon．Thedecolorizationtechnologyofpolysaccharidesactivecarbonwasoptimizedusingorthogonalexperimentwithtemperature，timeandthecontentofactivecarbonasfactorsandthecontentofpolysacch

5、aridesandthedecoloringrateasindexes．Theoptimaldecolorizationtechnologywasasfollows：temperature：40℃：thecontentofactivecarbonwas2％，andthestirringtimewas30min，thedecolorationtechnologyisapplicableforindustrializedproduction．ThepolysaccharideofchinesechestnuthasthreeGPCpeak，i

6、tsMwwere7．6min，2．31×10’Da，13．4min，3．71×103Da，14．5min．6．36x102Da．3．Thestudyresultsshowthatthepolysaccharidescontentsindifferentvarieties(families)aresignificantlydifferent；thecontentsinYanlong，YanshanzaofengandDaomazhaichestnutarerelativelyhigher．4．Thestudyresultsshowthatt

7、hetotalsugarcontentsindifferentvarieties(families)aresignificantlydifferent，thecontentsinYanlong，andXiahexi6andIIIDabanhongchestnutarerelativelyhigher．Thestudyresultsshowthatthereducingsugarcontentsindifferentvarieties(families)aresignificantlydifferent，thecontentsinYanlo

8、ng，andQianxi5chestnutarerelativelyhigher．Thestudyresultsshowthatthestarchcontentsindifferentvari