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1、阿拉伯糖-6-C-葡萄糖苷、芹菜素芸香糖苷、山奈酚六碳糖苷3种化合物为首次在无花果叶中发现。关键词：无花果；黄酮；超声波提取法；纯化；抗氧化活性；液质联用iiStudyonExtractionandPurificationProcess,Structural
IndentificationandtheAntioxidantActivityofFlavonoid
inFigLeaves(FicuscaricaL.)AbstractByusingleavesoffig(FicuscaricaLinn)asexperimentalmater
2、ials,theultrasoundassistedextraction(UAE)oftotalflavonoidswasstudied.Baseonthis,asinglefactorexperimentwasinvested.Thetechniqueenvironmentforseparatingandpurifyingthetotalflavonesoffigleavesbymacroreticularadsorbentswasevaluatedandmacroreticularadsorbentsresinwhichhadab
3、etterperformanceofadsorptionanddisadsorptioneffectwasfoundout.Theantioxidantabilitiesoffigleaveswerealsodetected.AtlasttheprincipalcompoundsofsolutionsoffigleaveswasanalyzedandidentifiedbyHPLC-MS.THEresultswereasthefollowings:Firstofall,theultrasoundassistedextraction(U
4、AE)oftotalflavonoidfromtheleavesoffigwasstudiedinthispaper.Second,tostudythetechniqueenvironmentforseparatingandpurifyingthetotalflavonesfromtheleavesoffigbymacroreticularadsorbentswerestudied.Third,theabilitiesoftotalflavonoidoffigleavesonscavenginghydroxylradical(·OH)
5、,superoxideanionradicals（O2-·）,andDPPHradicalsandreductioncapacityweredetected.Fourth,analyzedandidentifiedtheprincipalcompoundsinallofsolutionsoffigbyHPLC-MS.Theresultswereasthefollowings：1,Ultrasonicextractionprocessofthetotalflavoneswasoptimizedwithcentralcompositede
6、sign-responsesurfacemethodology.Inthisexperiment,thestudyfoundoptimalprocessofextractingtotalflavonoidfromfigleavesbyultrasonic.Inthemeanwhile,ittoldushowtoreusethefigleaves.Singlefactorexperimentswereinvestigated:ultrasonicextractiontime,ethanolconcentration,liquidextr
7、actionratethanthatoftheimpact.Then，responsesurfacemethodologydesignwasusedtodesignandevaluatetheexperiment.Practicalexamplesshowedthattheextractionratewas3.48%undertheoptimumextractingconditions:ethanol35.48%,ratioofsolidtoliquid(w∶v)1∶59,ultrasonicpower420W.2,Seventype
8、sofmacroporousadsorptionwereevaluatedforseparatingefficiencybymeasuringtheadsorptionratio,elutingratiooftotalf
