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1、2型糖尿病肾损伤大鼠模型的建立和肾脏CD34表达作者：胡波,李锋,李敏,王胜春【摘要】目的观察高脂喂养联合低剂量链脲佐菌素(STZ)注射的SD大鼠2型糖尿病肾损伤模型的代谢特征及CD34的变化。方法4周龄雄性SD大鼠60只随机分为2组。正常对照组10只,普通饲料喂养；糖尿病模型组50只,高脂饲料喂养,即普通饲料中加入1O%猪油、10%葡萄糖、1%胆固醇、2%蛋黄粉、1%胆酸钠。喂养4周后尾静脉注射STZ(35mg/kg)。所有大鼠在STZ注射10d与30d时做灌胃葡萄糖耐量试验(OGTT)。放免法测定血清胰岛素,免
2、疫组化染色观察肾脏CD34的阳性表达。结果糖尿病肾损伤大鼠空腹血糖(FBG)显著高于正常对照组(P<0.01),血糖值在注射完STZ后1～3个月比较稳定；24h尿蛋白从1个月后一直持续上升；空腹血清三酰甘油(TG)和游离脂肪酸(FFA)水平显著高于正常对照组(P<0.05)；尿素氮(BUN)、血肌酐(Cr)显著高于正常对照组(P<0.01)；在糖尿病肾损伤大鼠的肾脏中CD34出现高表达。结论高脂喂养联合低剂量STZ注射的糖尿病大鼠模型拟2型糖尿病肾病发生的主要病理生理过程,具有高血糖以及血脂异常和
3、肾功能损伤等基本特征；肾损伤可能与CD34有关。【关键词】糖尿病；大鼠；链脲佐菌素；CD34；动物模型 Abstract：ObjectiveToinvestigatethemetabolismcharacteristicsandCD34varianceofSDratsmodelswithtype2diabetesrenalinjurywithhighgreasefeedingandlow-doseSTZinjection.Methods60maleSDratswith4-weekoldwererandomized
4、intotwogroups：10inthecontrolgroupfeedingwithnormalforage,50intheDMgroupfeedingwithhighgreaseforage,whichconsistof10%lard,10%glucose,1%cholesterol,2%yolkpowder,and1%sodiumcholateaddingintothenormalfeeds.IntravenousinjectionofSTZafterfeeding4weeks(35mg/kg).Allth
5、eratsweretestedwithOGTTafter10daysand30daysinjectionofSTZ.Seruminsulinwasmeasuredbyradioimmunoassay(RIA)andimmunohistochemistrystainingwasadoptedtoobservethepositiveexpressionofrenalCD34molecular.ResultsFBGofratswithdiabetesandrenalinjurywassignificantlyhigher
6、thanthatinthecontrolgroup(P<0.01),bloodglucosestayedstable1-3monthsafterinjectionofSTZ,whilethe24hurineproteinkeptrisingafter1month,fastingserumtriglyceride(TG)andfreefattyacids(FFA)weresignificantlyhigherthanthatincontrolgroup(P<0.05),withsignificantlyh
7、igherbloodureanitrogen(BUN)andserumcreatinine(P<0.01).CD34showedhighexpressionininjuredkidneyoftheratswithdiabetes.ConclusionThemainpathologicalandphysiologicalprocessoftheoccurrenceoftype2diabetesmodelratswithhigh4greasefeedingandlow-doseSTZinjectionembody
8、thefundamentalcharacteristicsofhyperglycemia,bloodfatabnormityandrenalinjury.CD34mayberesponsiblefortherenalinjury. Keywords：diabetes；rats；STZ；CD34；animalmodel 胰岛素分泌缺陷和胰岛素抵抗所导致的体
