热休克蛋白27参与兔肺缺血预处理保护

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1、热休克蛋白27参与兔肺缺血预处理保护作者：涂军伟，徐正王万铁，吴成云，方舟溪作者单位：1.金华市中心医院呼吸内科，浙江金华321000;2.温州医学院病理生理学教研室，浙江温州325000;3.温州医学院附属第二医院呼吸内科;4.温州医学院电镜室【摘要】目的:探讨热休克蛋白27(HSP27)在肺缺血预处理保护中所起的作用及意义。方法：采用在体兔单肺原位缺血再灌注模型，30只日本大耳兔随机均分为三组：假手术(S)组、缺血/再灌注(I/R)组及缺血预处理(PC)组，实验检测肺组织湿干重比和肺泡损伤数比值，电镜评定肺组织超微结构损伤，免疫组化检测肺组织热休

2、克蛋白27的定位及表达。结果：①I/R组肺组织湿干重比(W/D)及肺泡损伤数比值(IAR)明显于S组(均P0.01);PC组W/D及IAR均显著低于I/R组(P0.05或P0.01)。②免疫组化显示PC组肺小动脉内膜及外膜层、肺小静脉壁全层、远端呼吸性细支气管及少许肺泡上皮HSP27有强表达;肺小静脉HSP27含量(平均吸光度值LD)高于I/R组和S组(均P0.01)。③I/R组肺组织超微结构的损伤明显，而PC组损伤明显减轻。结论：肺缺血预处理能减轻肺缺血再灌注损伤，HSP27表达增加并参与肺缺血预处理保护。【关键词】热休克蛋白;兔;缺血预处理;缺血

3、再灌注损伤Heatshockprotein-27participatedintheprotectionoflungischemicpreconditioninginrabbitTUJun-wei*，XUZheng-xie，WANGWan-tie，WuCheng-yun，FangZhou-xi.*DepartmentofRespi-ratoryofJinhuaCentralHospital，Jinhua，321000Abstract:Objective:Toinvestigatetheeffectandsignificanceofheatshockpr

4、otein-27intheprotectionaffordedbylungischemicpreconditioninginrabbits.Methods:Singlelunginsituischemicreperfusionanimalmodelwasused.30rabbitswererandomlydividedintothreegroups:sham(S)group，ischemic/reperfusion(I/R)groupandischemicpreconditioning(PC)group.Wettodryratio(W/D)andin

5、juredalveolirate(IAR)weredetermined.Ultramicroscopicstructuralchangewasobservedunderelectronmicroscope.Heatshockprotein-27wasdetectedwithimmunohistochemistry.Results:①W/DandIARinI/RgroupwerehigherthanthatinSgroup(P0.01).TheW/DandIARinPCgroupwerelowerthanthatinI/Rgroup(P0.05，P0.

6、01respectively).②Theheatshockprotein-27stronglyexpressedinintimaandextimaofsmallpulmonaryartery，vesselwallofsmallpulmonaryvein，respiratorybrochioleandpartofalveolarepithelium.Thelightdensityofheatshockprotein-27insmallpulmonaryveinsinPCgroupwashigherthanthatofothergroups.③Elect

7、ronmicroscopeshowedseriouslunginjuryoccurredinI/RgroupwhiletherewasnosignificantinjuryinPCgroup.Conclusion:Lungischemicpreconditioningcanmitigatelungischemiareperfusioninjury，highlyexpressedheatshockprotein-27participatedintheprotectionaffordedbylungischemicpreconditioning.Keyw

8、ords:heatshockprotein;rabbit;ischemicpreconditioning;i