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1、健脾止动汤对多发性抽动症模型鼠纹状体多巴胺通路的影响作者：卫利,王素梅,岳广欣,吴力群,郝宏文,张婷,陈自佳,刘岩,胡利军【摘要】目的研究健脾止动汤对多发性抽动症(TS)模型鼠纹状体多巴胺通路的影响,为健脾止动汤干预小儿TS提供神经生化学依据。方法90只雄性ICR小鼠随机分成6组,即空白组、模型组、泰必利组、健脾止动汤组、六君子汤组、泻青汤组。除空白组外,其余5组小鼠腹腔注射亚氨基二丙腈建立TS小鼠模型。除空白组和模型组给予生理盐水外,其余4组分别灌胃给予相应药物。4周后采用高效液相色谱电化学法测定TS模型鼠脑纹状体单胺类递质的含量。结果TS模型鼠表现出抽动症特征性的运动行为和刻板
2、行为。小鼠脑纹状体单胺类递质含量,模型组较空白组脑多巴胺(DA)、二羟苯乙酸(DOPAC)、高香草酸(HVA)、5-羟色胺(5-HT)、去甲肾上腺素(NE)含量无明显变化(P＞0.05)；泻青汤组较模型组DA、DOPAC含量降低(P＜0.05),泻青汤组较泰必利组脑DA、5-羟吲哚乙酸(5-HIAA)、NE含量降低(P＜0.01或P＜0.05),泻青汤组较六君子汤组脑DA、5-HIAA、NE含量降低(P＜0.01或P＜0.05)。结论健脾止动汤的主要作用组分为泻青汤,其抗抽动作用可能与兴奋脑纹状体多巴胺间接通路、降低DA含量有关。【关键词】多发性抽动症；纹状体；多巴胺通路；健脾止动
3、汤；小鼠11Abstract：ObjectiveTostudytheeffectsofJianpizhidongdecoctionondopaminepathwayinTSmodelmicestriatum,andprovideneuralbiochemicalproofforJianpizhidongdecoctionintreatingchildrenTourettesyndrome(TS).Methods90maleICRmicewererandomlydividedinto6groups,namelyblankcontrolgroup,modelgroup,Tiapride
4、group,Jianpizhidongdecoctiongroup,Liujunzigroup,Xieqinggroup.Exceptblankcontrolgroup,other5groupsmiceweremadetoTSmodelsbyintraperitonealinjectionof3,3’-Iminodipropionitrile(IDPN).Withtheexceptionofblankandmodelgroups,other4groupsweregivencorrespondingdrugsbyintragastricadministrationrespective
5、ly.4weekslater,thelevelsofmonoamineneurotransmitterinbrainstriatumofTSmodelmiceweredetectedbyhighperformanceliquidchromatographyandelectrochemicaldetection.ResultsTSmodelmiceappearedcharacteristiclocomotionbehaviorandstereotypybehavior.Thelevelsofmonoamineneurotransmitterswiththeexceptionof5-H
6、IAAhadnodifferenceinstriatumofmicebetweenTSmodelgroupandblankcontrolgroup(P＞0.05).Comparedwithmodelgroup,levelsofDAandDOPACinstriatumreducedinXieqinggroup(P＜0.05).ComparedwithTiapridegroup,levelsofDA,115-HIAA,NEinstriatumreducedinXieqinggroup(P＜0.01,P＜0.01,P＜0.05).ComparedwithLiujunzigroup,lev
7、elsofDA,5-HIAA,NEinstriatumreducedinxieqinggroup(P＜0.01,P＜0.01,P＜0.05).ConclusionsThemainlycomponentofJianpizhidongdecoctionisXieqingdecoction,thefunctionofimprovingsymptomsofTSmayberelatetoactivateindirectdopaminepathwayanddecreaseleve
