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1、基质细胞衍生因子1在骨髓间质干细胞归巢到急性梗死心肌中的作用2007年6月第33卷第6期Jian~suMedJ,June007'13310.基质细胞衍生因子1在骨髓间质干细胞归巢到急性梗死心肌中的作用庄瑜陈鑫石开虎徐明【摘要】目的探讨基质细胞衍生因子1(SDF_1)在骨髓问质干细胞(MSCs)归巢到急性梗死心肌中的作用.方法以免疫组化检测SDF-1在心肌梗死1,2,4,7,14,28d后的大鼠及非心肌梗死大鼠心肌中的表达差异;于心肌梗死4d后予以SDF-1,抗SDF-1抗体进行干预,同时经尾静脉注射BrdU标记的MSCs,3d后检测
2、梗死区MSCs的归巢量,28d后检测大鼠心功能状况,以及梗死区及其周围的血管密度;另设心肌梗死及非心肌梗死阴性对照组.结果SDF-1在心肌梗死后第1天即升高并达到峰值,以后逐渐下降,第14天恢复至正常水平.心肌梗死组干细胞归巢量明显大于非心肌梗死对照组.SDF-1处理组大鼠心肌梗死区干细胞归巢量,心功能水平及血管密度大于抗SDF-1抗体处理组及心肌梗死对照组(P<O.01).结论心肌梗死后早期梗死区SDF-1表达水平升高;SDF-1能够促进MSCs归巢并改善心功能,促进毛细血管增生可能为其改善心功能的机制之一.【关键词】心肌梗
3、死;骨髓间质干细胞;归巢;心功能;基质细胞衍生因子一1【中图分类号1R541【文献标识码】A【文章编号10253—3685(2007)06—0579—04TheefofsI,F-1onmy瑚IsteIIlIlsl10IIliI-gtomyocardialin瞄siteZNGy",CttF_,NXin,SHlKaihu,eta1.DepartmentofThoracic&CardiovascularSurgery,AffiliatedNanjingFirstHospital,Nan]ingMedicalUniversity,N
4、aing210006,CHINA[AStract]ObjectiveToinvestigatetheeffectofstromalcell-derivedfactor-1(SDF-1)onmesenchymalstemcells(MSCs)homingtomyocardialinfarctsiteMethodsSDF-1expressionwasmeasuredbyimmunohistochemistryinshamoperatedorinfarctedheartsonthe1st,2nd,4th.7th,14thand28thda
5、yaftermyocardialinfarction(MI).MSCsfromdonorratswere1abeledwithBrdU.)F_1,anti-SDF-1antibodyorsalinewasinjectedintoperi-infarctmyocardium4daysafterMI.Then.atotalof5×10cellsin2.5m10fPBSorequalvolumePBSalonewereinjectedthroughthetai1veimThenumberoflabeledMSCsintheinfarcte
6、dheartswascotmted3daysafterinjection.Cardiacfunctionandbloodvesseldensitywereassessed28daysafterinjection.ResultsMyocardialS】)F_1expressionincreasedandpeakedonthefirstdayafterM1anddecreasedthereafter.whichremainedunchangedinshamoperatedhearts.TheMSCsinthehostheartswere
7、moreabundantintheMIgroupsthanthoseinthenon-MIgroup.whichweremoreintheSDF.1injectedgroupthanthoseintheanti-sDF-1antibodyandsalineiniectedgroups(P<0.05).CardiacfunctionwasimprovedmoreinSDF-1injectedgroupthanthatinanti—SDF-1antibodyandsalineinjectedgroups(P<0.01).Ne
8、ovascularizationinSDF-iectedgroupsignificantlyincreasedthanthatintheothergroups(P<0.01).ConclusionMyocardialSDF-1e
