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时间:2017-12-08
《sirna干扰整合素β4的表达对mda-mb-231乳腺癌细胞侵袭和迁移的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、HainanMedJ,Dec.2013,Vo1.24,No.23海南医学2013年l2月第24卷第23期doi:10.3969/j.issn.1003—6350.2013.23.1430siRNA干扰整合都4的表达对MDA—MB一231乳腺癌细胞侵袭和迁移的影响曹颖,吴倩,文建力,童英(贵州省人民医院病理科,贵州贵阳550002)【摘要】目的采用RNA干扰(SmallinterferenceRNA,siRNA)技术抑制MDA.MB一23l乳腺癌细胞中整合素(integrin/34)基因表达,观察其对细胞侵袭和迁移能力的影响。方法选用MDA.MB一231
2、乳腺癌细胞为研究对象,应用siRNA技术沉默癌细胞Integrin/34mRNA的表达,Rea1.timePCR和Westernblotting方法分别检测未转染Integrin/34siRNA的乳腺癌细胞组(简称空白对照组)及转染Integrin/34siRNA的乳腺癌细胞组(简称Integrin/34RNA干扰组)的Integrin/34mRNA和蛋白表达水平;Transwell实验检测两组细胞的侵袭及迁移能力。结果与空白对照组相比,Integrin/34RNA干扰组Integrin/34的mRNA及蛋白表达水平分别降低了86%(P,0.01)和8
3、9%(P4、-231cellline.CAOYing,uQian,WENJian-li.TONGYing.DepartmentofPathology,Gu~houProvinc&lPeopleHospital,Guiyang550002,Guizhou,CHINA【Abstract】ObjectiveToinvestigatetheinfluenceofinhibitedintegrin/34bysmallinterferenceRNA(siRNA)ontheabilityofinvasionandmigrationinbreastcancerMDA-MB一2315、cellline.MethodsThebreastcancerMDA-MB一231celllineswereselected.Integrin/34genewassilencedbyRNAinterferencetechnique,theexpressionsofintegrin/34atmRNAandproteinlevelsinMDA—MB—231cellsnottransfectedwiththeintegrin/34(blankcontrols)andMDA-MB一231cellstransfectedwiththeintegrin/34siR6、NA(integrin/34siRNA)werestudiedbyreal—timePCRandwesternbloting,respectively.TheabilityofinvasionandmigrationweremeasuredthroughTranswel1.ResultComparedwiththeblankcontrols,theexpressionsofintegrin/34atmRNAandproteinlevelsinintegrin/34siRNAweredecreasedby86%(P<0.01)and89%(P<0.oi)7、,respectively,andtheabilityofinvasionandmigrationwerealsodecreasedfP8、grin/34;Invasion;Migration乳腺癌是严重危害女性身心健康的常见恶性肿键
4、-231cellline.CAOYing,uQian,WENJian-li.TONGYing.DepartmentofPathology,Gu~houProvinc&lPeopleHospital,Guiyang550002,Guizhou,CHINA【Abstract】ObjectiveToinvestigatetheinfluenceofinhibitedintegrin/34bysmallinterferenceRNA(siRNA)ontheabilityofinvasionandmigrationinbreastcancerMDA-MB一231
5、cellline.MethodsThebreastcancerMDA-MB一231celllineswereselected.Integrin/34genewassilencedbyRNAinterferencetechnique,theexpressionsofintegrin/34atmRNAandproteinlevelsinMDA—MB—231cellsnottransfectedwiththeintegrin/34(blankcontrols)andMDA-MB一231cellstransfectedwiththeintegrin/34siR
6、NA(integrin/34siRNA)werestudiedbyreal—timePCRandwesternbloting,respectively.TheabilityofinvasionandmigrationweremeasuredthroughTranswel1.ResultComparedwiththeblankcontrols,theexpressionsofintegrin/34atmRNAandproteinlevelsinintegrin/34siRNAweredecreasedby86%(P<0.01)and89%(P<0.oi)
7、,respectively,andtheabilityofinvasionandmigrationwerealsodecreasedfP8、grin/34;Invasion;Migration乳腺癌是严重危害女性身心健康的常见恶性肿键
8、grin/34;Invasion;Migration乳腺癌是严重危害女性身心健康的常见恶性肿键
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