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1、天津医药2012年6月第40卷第6期537doi:10.3969/j.issn.0253-9896.2012.06.003双丁酰环腺苷酸对甲状腺鳞癌SW579细胞株增殖的影响*△高月肖建英赵颂张秀梅刘超王翠瑶刘洋摘要目的:探讨双丁酰环腺苷酸(dbcAMP)对甲状腺鳞癌SW579细胞株增殖的影响。方法:将dbcAMP0.5、1.0、2.0mmol/L作用于甲状腺鳞癌SW579细胞24h后,用四甲基偶氮唑蓝比色(MTT)法检测SW579细胞增殖的变化;经dbcAMP0.5mmol/L作用24h后,ELISA法检测细胞成熟促进因子(MPF)的活性,WesternBlot方
2、法检测cdc2-Tyr15磷酸化水平,流式细胞术检测细胞周期变化情况。结果:与对照组相比,经不同浓度dbcAMP处理的SW579细胞株的增殖均受抑制。在0.5mmol/LdbcAMP作用下24h,SW579细胞MPF活性为(22.87±2.57)ng/L,远小于对照组的(115.50±7.53)ng/L,差异有统计学意义(t=3.324,P<0.05)。实验组cdc2-pTyr15磷酸化水平(0.258±0.026)高于对照组的(0.142±0.009),差异有统计学意义(t=7.424,P<0.05)。与对照组相比,应用0.5mmol/LdbcAMP处理细胞之后,
3、G0/G1期无显著变化,S期细胞数量减少,G2/M显著增多。结论:dbcAMP能够显著降低甲状腺鳞癌SW579细胞株的活力,使细胞增殖受到明显抑制。关键词环AMP甲状腺肿瘤癌细胞增殖dbcAMPEffectsofdbcAMPontheProliferationofSW579CellLineGAOYue,XIAOJianying,ZHAOSong,ZHANGXiumei,LIUChao,WANGCuiyao,LIUYangDepartmentofBiochemicalandMolecularBiology,LiaoningMedicalCollege,Jinzhou1
4、21001,ChinaAbstractObjective:TostudytheeffectofdualdibutyrylcyclicAMP(dbcAMP)ontheproliferationofSW579cellline.Methods:ThegrowthactivityofcellsweredetectedbyMTT,afterSW579cellsweretreatedwithdbcAMP(0.5,1.0,2.0mmol/L)for24h.Atthelevelof0.5mmol/L,theactivityofmaturationpromotingfactor(MP
5、F)weretestedthroughELISA.Theproteinexpressionofthephosphorylationlevelofcdc2-Tyr15wasmeasuredbyWesternblot.Changesofthecellcyclewerede⁃tectedbyflowcytometry.Results:Comparedwithcontrolgroup,thegrowthactivityofSW579cellswasreducedaftertreatmentwithdifferentlevelsofdbcAMP.ResultsofELISAs
6、howedthattheactivityofMPFwas(22.87±2.57)ng/Laftertreatmentwith0.5mmol/LdbcAMPfor24h,whichwassignificantlylowerthanthatofcontrolgroup(115.5±7.53)g/L(t=3.324,P<0.05).Theproteinexpressofthephosphorylationlevelofcdc2-Tyr15washigherintestgroup(0.258±0.026)thanthatofcontrolgroup(0.142±0.009,
7、t=7.424,P<0.05).Comparedwithcontrolgroup,thenumberofSW579cellshadnosignificantdifferenceinG0/G1phase,reducedintheSphaseandincreasedobviouslyintheG2/Mphaseaftertreatingwith0.5mmol/LdbcAMP.Conclu⁃sion:dbcAMPcouldsignificantlyreducethevitalityofSW579cells,bywhichthecellproliferationwass