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1、ArchVirol(2000)145:1725–1732Oralimmunogenicityoftheplantderivedspikeproteinfromswine-transmissiblegastroenteritiscoronavirusBriefReportN.Gómez1,A.Wigdorovitz2,S.Castañón3,F.Gil1,R.Ordás3,M.V.Borca2,andJ.M.Escribano11DepartamentodeMejoraGenéticayBiotec
2、nologí,INIA,Madrid,Spain2InstitutodeVirología,CICV,INTA-Castelar,BuenosAires,Argentina3DepartamentodeBiologíadeOrganismosySistemas,InstitutoUniversitariodeBiotecnologíadeAsturias(CSIC),UniversidaddeOviedo,Oviedo,SpainAcceptedMarch7,2000Summary.Transge
3、nicplantsrepresentaninexpensivealternativetoclassicalfermentationsystemsforproductionofrecombinantsubunitvaccines.TransgenicpotatoplantswerecreatedthatexpresstheN-terminaldomainoftheglycoproteinS(N-gS)fromTransmissiblegastroenteritiscoronavirus(TGEV),
4、containingthemajorantigenicsitesoftheprotein.ExtractsfrompotatotubersexpressingN-gSwereinoculatedintraperitoneallytomice,andthevaccinatedmicedevelopedserumIgGspecificforTGEV.Furthermore,whenpotatotubersexpressingN-gSwerefeddirectlytomice,theydevelopeds
5、erumantibodiesspecificforgSprotein,demonstratingtheoralimmunogenicityoftheplantderivedspikeproteinfromTGEV.*Transmissiblegastroenteritiscoronavirus(TGEV)isthecausativeagentofacutediarrheaofnewbornpigletsthatprovokeshighratemortalitiesinaffectedfarms.Pr
6、otectiveimmunityagainstthisdiseasehastobedevelopedinpregnantsowsinordertoconferpassiveprotectiontothepigletstroughcolostrumandmilk.NeutralizingantibodiesagainstthevirusaredirectedmainlytoglycoproteinS[12,15],andrelevantepitopesinneutralizationhavebeen
7、mappedintotheN-terminaldomainofthisprotein[8].FourmajorantigenicsiteshavebeendescribedintheglobularpartofglycoproteinS(gS),ofwhichsiteAistheimmunodominant[9–11,21].TheprotectionofsucklingpigletsagainstanentericTGEVinfectionisbasedontheuptakeofspecificl
8、actogenicantibodies,mainlyoftheIgAclass,inthemilk1726N.Gómezetal.ofTGEV-immunemothers[4].Sincetheconceptofvaccineproductionintrans-genicplantswasfirstintroducedbyMasonandco-workersin1992[16],theoralimmunogenicityofseveralantigensderivedfromhuma
