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1、Bundesgesundheitsbl-Gesundheitsforsch-GesundheitsschutzLeitthema:PerspektivenzurEntwicklungder,,GrünenGentechnik”2000·43:110–115©Springer-Verlag2000J.M.Escribano1·M.V.Borca21DepartamentodeMejoraGenéticayBiotecnología,Madrid2InstitutodeVirología,BuenosAiresImmunogenicityofarecombin
2、antcoronavirusspikeglycoproteinexpressedintransgenicplantsSummaryKeywordsTheconceptofvaccineproductionintransgenicplantswasfirstintroducedbyRecently,ithasbeendemonstratedthatGeneticallymodifiedplant·Vaccine·Masonandco-workersin1992[8].Pro-plantsofferthepossibilityofproducinglowCor
3、onavirus·TGEVteinsinvolvedinprotectiveimmunere-costsubunitvaccinesthatcanbeeitherpa-sponsecanbeproducedatalowcostrenterallyororallyadministered.Herewere-andeasilypurifiedfromplantextractsviewdataweobtainedontheimmunologi-forparenteralinoculation.Inaddition,calresponseelicitedbytwo
4、recombinantoralimmunizationbyediblevaccinesversionsoftheglycoproteinSfromtheswi-producedintransgenicplantscouldnetransmissiblegastroenteritiscoronavirusSwinetransmissiblegastroenteritisstimulateimmuneresponsesatthepor-(TGEV)expressedintransgenicplants.Ara-virus(TGEV)isthecausative
5、agentoftalofentranceformanypathogens,fa-bidopsisorpotatoplantsweregeneticallyacutediarrheaofnewbornpigletsthatcilitatingthedesignoflarge-scaleim-transformedwithcDNAsconstructsenco-provokeshighratemortalitiesinaffect-munizationprograms.ThepresenceofdingtheN-terminaldomain(aaresidue
6、sedfarms.Protectiveimmunityagainstspecificantigensinplants,evenatlow1–750)orthefull-lengthglycoproteinSofthisdiseasehastobedevelopedinlevels,canraiseimmunereactionsbytheTGEV,responsiblefortheneutralizinganti-pregnantsowsinordertoconferpas-oralroutecomparabletoconventionalbodyinduc
7、tionagainstthevirus,underthesiveprotectiontothepigletstroughco-vaccines[9,10,11].controlofthecauliflowermosaicvirus35Slostrumandmilk.Neutralizinganti-Today,hepatitisBsurfaceantigen(CaMV35S)promoter.GenomicDNAandbodiesagainstthevirusaredirected[12],E.coliheat-labileenterotoxin(LT-m
8、RNAanalysisofleaveextractsfromtra
