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1、JournalofGeneralVirology(1996),77,309-313.PrintedinGreatBritain309ShortcommunicationAnadenovirusrecombinantexpressingthespikeglycoproteinofporcinerespiratorycoronavirusisimmunogenicinswinePaulCallebaut,~*LuisEnjuanes2andMauricePensaerP1LaboratoryofVeterinaryVirology,FacultyofVeter
2、inaryMedicine,UniversityofGhent,Salisburylaan133,B-9820Merelbeke,Belgiumand~CentroNacionaldeBiotecnologia,CSIC,CampusUniversidadAut6noma,CantoBlanco,E-28049Madrid,SpainThefull-lengthspike(S)geneofporcinerespiratorycomigratingwiththeauthenticprecursorS'andthecoronavirus(PRCV)wasins
3、ertedintothegenomeofmatureSproteinofPRCV,respectively.Theextra-humanadenovirustype5downstreamoftheearlycellularrecombinantantigencorrespondedtothetranscriptionregion3promoter.Therecombinantvirus175kDamatureprotein.SomerecombinantSproteinreplicatedinculturesoftheswinetesticleSTcell
4、linewasexposedonthecellsurfaceandwasrecognizedbyanddirectedthesynthesisofSantigenwithamaximumneutralization-mediatinganti-Smonoclonalantibodies.yieldofapproximately26~tgper106cells.TheantigenPiglets,inoculatedoronasaUywiththerecombinantwascell-associatedexceptinthelatephaseofthead
5、enovirusvectordevelopedPRCV-neutralizingseruminfection,whenasmallamount(3'5lagper106cells)wasantibodiesandwerepartiallyprotectedagainstPRCVreleased.Thecell-associatedantigenconsistedofpoly-challenge,demonstratingthepotentialofliveadenoviruspeptidesofmolecularmass160kDaand175kDa,as
6、vaccinevector.Porcinerespiratorycoronavirus(PRCV)(Pensaertetal.,beingtransportedtothecellsurface,withoutthe1986)belongstotheCoronaviridae,afamilyofen-cooperationofotherviralproteins(Pulford&Britton,veloped,positive-strandedRNAviruses.Itisanti-1991;Godetetal.,1991).Sincecellsurface
7、expressiongenicallyandgeneticallycloselyrelatedtoentero-hasbeenreportedtoenhancetheimmunogenicityofpathogenictransmissiblegastroenteritisvirus(TGEV)ofsomeantigens(Andrewetal.,1990),allofthissuggestsswine(Callebautetal.,1988;Rasschaertetal.,1990;thattheSglycoproteinmaybeausefulanti
8、genfortheSanchezetal.,1992)butmai
