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1、JVetDiagnInvest8:241-244(1996)UseofnonradioactivecDNAprobestodifferentiateporcinerespiratorycoronavirusandtransmissiblegastroenteritisvirusisolatesEricM.Vaughn,PatrickG.Halbur,PremS.PaulPorcinerespiratorycoronavirus(PRCV),amemberoftheinanucleicacidhybridizationtodifferentiatebetweenPRCVfamilyCoron
2、aviridae,isantigenicallyrelatedtotransmis-andTGEVisolates.WehavedevelopedtwocDNAprobes3,4,6,9,12,17,18,20,24siblegastroenteritisvirus(TGEV)ofswine.onthebasisofthenucleotidesequenceoftheSgeneofTGEV3,9,13,23PRCV,nowthoughttobeamutantofTGEV,wasfirstthatcanbeusedtodifferentiatebetweenPRCVandTGEV1220is
3、olatedin1984frompigsinBelgiumthatwereseropositiveisolates.OtherresearchershavereportedontheuseofTGEVforTGEVbutdidnothaveahistoryofclinicaltransmissiblecDNAprobesinanucleicacidhybridizationtodifferentiategastroenteritis(TGE).SincetheinitialisolationofPRCV,itbetweenTGEVandPRCVisolates;however,thesep
4、revi-hasbeenfoundthatinfectionsofswineinEuropewithPRCVouslyreportedhybridizationmethodsreliedonusingcDNA4,8,9321,8,16,22arewidespread.PRCVhasalsobeenisolatedinswineinprobeslabeledwiththeradionuclideP.Inthisarticle7,11,20,24theUnitedStates,butitsprevalenceinherdswithinwereportonanucleicacidhybridiz
5、ationassaythatusesantheUnitedStatesisnotknown.immunochemiluminescentdetectionmethodtodifferentiateThereareseveralsimilaritiesbetweenPRCVandTGEV.betweenTGEVandPRCVisolates,thusavoidingtheuseofBothviruseshave3majorstructuralproteins:thesurfaceradioactivematerials.spike(S)glycoprotein,theintegralmemb
6、raneglycoprotein,TheisolationofthePRCVisolatesAR310,LEPP,and3,9,13,2420andaninternalnucleoprotein.NucleotidesequencesofIA1894hasbeenpreviouslydescribed.ThePRCVisolatesPRCVisolatesthusfarstudiedshowthattheyarecloselyPON,IA725,andIA588wereisolatedfromnasalswabsfrom3,13,23relatedtoTGEVbutthatthereare
7、somestrikingdiffer-swineinherdsthathadantibodiestoTGEVbutnoevidenceences.PRCVisolateshaveacharacteristicdeletioninthe5'ofdiarrheaandthusweresuspectedofhavingPRCVinfec-3,8,13,20,23endoftheSgenewhencomparedtoTGEV,a
