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1、_ArchivesArchVirol(1992)125:193-204Virology©Springer-Veflag1992PrintedinAustriaComparisonofhemagglutinating,receptor-destroying,andacetylesteraseactivitiesofavirulentandvirulentbovinecoronavirusstrainsJ.Storz1,X.M.Zhang1,andR.Rott31DepartmentofVeterinaryMicr
2、obiologyandParasitology,SchoolofVeterinaryMedicine,LouisianaStateUniversity,BatonRouge,Louisiana,U.S.A.aInstitutfiirVirologie,Justus-LiebigUniversitfit,Giessen,FederalRepublic:ofGermanyAcceptedNovember29,1991Summary.Hemagglutinatingandacetylesterasefunctions
3、aswellasthe124kDaglycoproteinwerepresentinthehighlycell-cultureadapted,avirulentbovinecoronavirusstrainBCV-L9,intheNordenvaccinestrainderivedfromit,andin5wild-type,virulentstrainsthatmultipliedinHRT-18cellsbutwererestrictedinseveraltypesofculturedbovinecells
4、.TheBCV-L9andthewild-typestrainBCV-LY-138agglutinatedchickenandmouseerythrocytes.Theacetylesterasefacilitatedbreak-downoftheBCV-erythrocytecomplexwithchickenbutonlytoaminimalextentwithmouseerythrocytesinthereceptor-destroyingenzymetest.Purifiedpreparationsof
5、thevaccineandthewild-typestrainsagglutinatedchickenerythrocytesatlowtitersandmouseerythrocytesat128to256timeshighertiterswhereasreceptordestroyingenzymeactivitywasdetectableonlywithchickenerythrocytes.Whenwild-typestrainswerepropagatedinHRTcellsatlowpassagel
6、evels,theyproduced5x105to4.5x106plaqueformingunitsper50glwhichagglutinatederythrocytesfrommicebutnotfromchickens.Diisopropylfluoro-phosphatemoderatelyincreasedthehemagglutinationtiters,butcompletelyinhibitedthereceptordestroyingenzymeofpurifiedvirusofallstra
7、ins.Ithadvirtuallynoinfuenceontheplaque-forminginfectivityofthedifferentBCVstrains.TheacetylesteraseofstrainBCV-L9reactinginthereceptor-destroyingenzymetestwasstablefor3hat37and42"C.Itwasinactivatedwithin30minat56°Cwhilethehemagglutininfunctionofthisstrainwa
8、sstablefor3hat37,42,and56°C,butitwasinactivatedat65°Cwithinlh.IntroductionBovinecoronavirus(BCV)hasahemagglutinin(HA)forsomeerythrocyteswithanapproximatemolecularmassof62kDaand124kDainthereduced
