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《冠状病毒Coronavirusl论文-1994 Biological and genetic characterization of a hemagglutinating coronavirus isolated from a diarrhoeic child.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、JournalofMedicalVirology44152-161(1994)BiologicalandGeneticCharacterizationofaHemagglutinatingCoronavirusIsolatedFromaDiarrhoeicChildX.M.Zhang,W.Herbst,K.G.Kousoulas,andJ.StorzDepartmentofVeterinaryMicrobiologyandParasitology,SchoolofVeterinaryMedicine,Louisia
2、naStateUniversity,BatonRouge,Louisiana(X.M.Z.,K.G.K.,J.S.);andZnstitutfurHygieneundInfektionskrankheitenderTiere,Justus-LiebigUniversityGiessen,Giessen,FederalRepublicofGermany(W.H.)ThecoronavirusstrainHECV-4408wasisolatedity,antigenicandnucleotidese-fromdiarr
3、heafluidofa6-year-oldchildwithquencecomparisonsacutediarrheaandpropagatedinhumanrectaltumor(HRT-18)cells.Electronmicroscopyre-vealedcoronavirusparticlesinthediarrheafluidINTRODUCTIONsampleandtheinfectedHRT-18cellcultures.Thisviruspossessedhemagglutinatingandac
4、et-Coronavirusesareimportantcausesofgastroenteri-ylesteraseactivitiesandcausedcytopathicef-tisinanimals,buttheirinvolvementinhumanentericfectsinHRT-18cellsbutnotinMDBK,GBKanddiseasesremainsenigmaticwithevidencelimitedtoFEcells.OneoffourS-specificmonoclonalanti
5、-electronmicroscopicdetectionofcoronavirus-likepar-bodiesreactedinWesternblotswithHECV-4408,ticlesinstoolsamplesobtainedfrompatientswithBCV-L9andBCV-LY138butnotwithHCV-OC43,acutegastroenteritisornecrotizingenterocolitis.At-andtworeactedwithBCV-L9butnotwithHECV
6、-temptstopropagatethesevirusesincellculturesre-4408,BCV-LY138andHCV-OC43.OneS-specificmainedfutilewhileimmunologicalstudiesindicatedandtwoN-specificmonoclonalantibodiesre-thattheyarecoronaviruses[Kiddetal.,1989;Marshallactedwithallofthesestrains.cDNAencompass-
7、etal.,1989;Singhetal.,1989;Battagliaetal.,1987;ingthe3'8.5kboftheviralRNAgenomewasHuang,1987;Schnagletal.,1987;Gernaetal.,1985;isolatedbyreversetranscriptionfollowedbyMortensenetal.,1985;Sitbon,1985;MacNaughtonpolymerasechainreactionamplificationhadsizeandDavi
8、es,1981;Mathanetal.,19751.AnepidemicofandrestrictionendonucleasepatternssimilartonecrotizingenterocolitisinchildrenofaspecialcarethoseofBCV-L9andBCV-LY138.Incontrast,thenurseryinah
