QIAgen病毒DN;RNA共提取试剂盒QIAamp-MinElute-Virus-Spin-Handbook.pdf

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1、1063023_HB19.04.201014:49UhrSeite1ThirdEditionApril2010QIAamp®MinElute®VirusSpinHandbookForsimultaneouspurificationofviralRNAandDNAfromplasma,serum,andcell-freebodyfluidsSample&AssayTechnologies1063023_HB19.04.201014:49UhrSeite2Trademarks:QIAGEN®,QIAamp®,QIAcube®,BioRobot®,EZ1™MinElute

2、®(QIAGENGroup);Corex®(Corning,Inc.);Eppendorf®(Eppendorf-Netheler-HinzGmbH).©2002–2007QIAGEN,allrightsreserved.QIAGENisamemberoftheForestStewardshipCouncil(FSC).Fortheproductionofprintedmaterials,includinghandbooks,QIAGENhasapolicytoselectsuppliersthatcomplywithFSCstandardsforprintingp

3、rocessesandwell-managedforests.1063023_HB19.04.201014:49UhrSeite3ContentsKitContents4Storage4QualityControl5ProductUseLimitations5ProductWarrantyandSatisfactionGuarantee6TechnicalAssistance6SafetyInformation7Introduction8Principleandprocedure8Yieldandsizeofviralnucleicacids10CarrierRNA

4、12Additionofinternalcontrols12EquipmentandReagentstoBeSuppliedbyUser13ImportantNotes14HandlingofQIAampMinEluteColumns14Centrifugation14ProcessingQIAampMinEluteColumnsinamicrocentrifuge14PreparationofRNA15Samplestorage15PreparationofQIAGENProtease15AdditionofcarrierRNAtoBufferAL16Buffer

5、AW117BufferAW218Elutionofnucleicacids18ProtocolPurificationofViralNucleicAcidsfromPlasmaorSerum19TroubleshootingGuide22Appendix25OrderingInformation27QIAampMinEluteVirusSpinHandbook04/201031063023_HB19.04.201014:49UhrSeite4KitContentsQIAampMinEluteVirusSpinKit(50)Catalogno.57704Numbero

6、fpreps50QIAampMinEluteColumns50CollectionTubes(2ml)200BufferAL*12mlBufferAW1*(concentrate)19ml†BufferAW2(concentrate)13ml†BufferAVE(tubeswithpurplecaps)5x2ml†ProteaseResuspensionBuffer6mlCarrierRNA(tubeswithredcaps)310µg®‡QIAGENProtease1vialHandbook1*Containsachaotropicsalt.Takeappropr

7、iatelaboratorysafetymeasuresandweargloveswhenhandling.Notcompatiblewithdisinfectantscontainingbleach.Seepage7forsafetyinformation.†Containssodiumazideasapreservative.‡Resuspensionvolume1.4ml.See“PreparationofQIAGENProtease”,page15.StorageQIAampMinElutecolumnsshouldbestoredat2–8°Cupon