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1、Anovelmethodofdiffractionimagingflowcytometryforsizingmicrospheres11221,2,3SaYu,JunZhang,MarinaS.Moran,JunQ.Lu,YuanmingFeng,and2,1,Xin-HuaHu*1DepartmentofBiomedicalEngineering,TianjinUniversity,Tianjin300072,China2DepartmentofPhysics,EastCarolinaUniversity,Greenville,NorthCarolina27858,USA3ymf
2、eng@tju.edu.cn*hux@ecu.eduAbstract:Wereportanovelmethodofdiffractionimagingflowcytometrytomeasureandanalyzesizedistributionofmicrospheres.Anautomatedandrobustimageprocessingsoftwarebasedontheshort-time-Fourier-transformalgorithmhasbeendevelopedtoanalyzethecharacteristicandspatiallyvaryingoscil
3、lationsofsidescattersrecordedasadiffractionimage.Ourresultsdemonstratethatthenewmethodallowsaccurateandrapiddeterminationofsinglemicrospheres’diametersrangingfrom1to100μm.Thecapacityforanalysisoflightscatteringbytwo-sphereaggregateshasbeendemonstratedbutanalyticaltoolsforcharacterizationofaggr
4、egatesbymultiplemicrospheresremaintobedeveloped.©2012OpticalSocietyofAmericaOCIScodes:(290.5850)Scattering,particles;(110.1650)Coherenceimaging.Referencesandlinks1.K.K.Kim,D.W.Pack,M.Ferrari,A.P.Lee,andL.J.Lee,“Microspheresfordrugdelivery,”inBiologicalandBiomedicalNanotechnology,A.P.Lee,J.Lee,
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