舒郁胶囊及柴胡提取物含药血清对大鼠海马神经元gababr2介导的erk通路和ac%2fcamp%2fcreb通路的影响

《舒郁胶囊及柴胡提取物含药血清对大鼠海马神经元gababr2介导的erk通路和ac%2fcamp%2fcreb通路的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、异（P>0.05)，但柴胡组CAMP的含量有明显下调的趋势；给予GABAbR2拮抗剂干预的大鼠海马神经元P-ERK1/2水平较激动剂组显著降低（P〈0.01),舒郁组无显著降低，但是有降低趋势，柴胡组无降低趋势；给予舒郁胶囊血清组和拮抗剂组干预的大鼠海马神经元P-CREB水平显著降低（P〈0.05，P〈0.01)，柴胡组较激动剂组则无显著性差异。结论：1、釆用慢性温和应激法可以成功制备抑郁情绪大鼠模型，糖水偏好实验、旷场试验结合宏观行为药理学可有效评价模型。2、抑郁情绪大鼠模型各组血清来干预大鼠离体海马神经元后，大鼠海马神经元GABAbR2蛋白表达升高，表明GABAb

2、R2与抑郁情绪关系密切，可能是抑郁情绪发病的中枢机制之一。3、中药舒郁胶囊可以有效纠正模型GABAbR2蛋白表达异常升高的状态，并且可以降低海马神经元GABAbR2介导的信号通路活性，可能是该药治疗抑郁情绪的机制之一。关键词抑郁情绪；GABAbR2;舒郁胶囊；柴胡皂苷；海马神经元；信号通路丁heEffectofShuyuCapsuleandBupleurumExtractSerumonGABABR2-mediatedsignalpathwayinPrimaryCulturedRatHippocampusNeuronsSpecialty:PharmacologyofTr

3、aditionalChineseMedicineTutor:LingXueAuthor:Ying-fengJiangAbstractObjective:Ouraimwastoinvestigatetheproteinlevelsofv-aminobutyricacidB2(GABAbR2)receptorinrathippocampusneuronswiththemoddofratdepressivemoodserumintervention,tostudythecorrectingfunctionofShuYucapsuleandBupleurumextractwh

4、ichgivesapreliminarydiscussiononthepathogenesisofdepressionemotion,aswellasstudiestheeffectsofShuyucapsule；weactivatedthisreceptorusingGABAbR2specificityagoniststodetectcontainmedicine9erumwashowtoactivatetheeffectorlevelofchangesofdownstream,Ouraimwastoexplorethetransductionmechanismof

5、ShuYucapsuleonGABAbR2downstreamsignalpathway.Methods1、weusedthechronicmildstress(CMS)toestablishthedepressionemotionmodelinrats,andthentheratswereevaluatedbysucroseintake、thebodyweightandopen-fieldfrombeginningtotheendforthemodel.Serumofratsineachgroupwascollectedandthenaddedintothethem

6、ediumofvitroculturedhippocampiprimaryneurons.AtlasttheexpressionofGABAbR2andP-ERK1/2weredetectedwithVNfesternBlotrespectively.2、Combiningwithserumpharmacologyandcellculturetechnology,weactivatedthisreceptorusingGABAbR2specificityagonists,andobservedthechangeofthecAMPle*/elandtheexpressi

7、onofP-ERK1/2andP-CREB.Results1、Comparedwiththenormalgroup,theweight、thetotalscoreofopenfieldtestandthesucroseintakeofpr^erencetestofratsindepressionemotionmodelgrouplessenedsignificantly(P<0.01)；Ontheotherhand,comparedwiththedepressionemotionmodelgroup,theweight,thetotal9coreof