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ID:9290924
大小:949.67 KB
页数:8页
时间:2018-04-26
《z24经口染毒大鼠的血浆蛋白质组学分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、Z24经口染毒大鼠的血浆蛋白质缀学分析王莹1,2王全军1董廷生1袁本利1马华智’昊纯启’廖明阳4(1.警雾医学科学院瀑物药物礤究所,闽家北象努街蜜全浮价研究环f;心.北察100850;2.辽。尹医学院药物掰究所,辽宁镕翔121000)缡囊;秘瓣;邋{i堕臁浆畿搦凌组学分橇留栽z24翳F濠馕的潜谯生耪标塞麴艇瀑憋辩溺机麓游研髭线索。方浚;Wistar_犬飘鲶翻徐皆0、50、100嗣200m拶,kgZ245d膝取瞳帮躲组纵,避豁瓶浆生睨指梅耩N,£It羧辅壤誉糗豢,蘩溷敬囤电泳。疆谱控拳进行巍浆缀白矮缀攀分擀,臀袋用Westernblot拨
2、拳瓣‘趣浆鼢滤澄国B熊表达进挂骏涯。翁裂:100、200mg/kg组舷鏊爰冬叛酸转铤酶,碱性礴陵黪、憝貔筑漾,协濑篓黼,憨飘麟簿嚣藉,疆豢瓤蹲嘏;50mg/kg缀总麟弱孵开离,磁豪鬣下降:Z24器娥鞠滋现琢闷鞣竣的孵绷魁空泡变{!;li簌纤维缀娥臻t‰豢隐矮镦学舔究鹾啄7_24作用詹六鼠疯浆哮一齄媾鬣翻B簿浚这。F调,鹩甄陵健虢璃黢念娥懿ASS)、蕊裳缀翻篱体、黪仑踩缀翻簿灌达.I:调;Westernblot绻浆tg鼹攀Z24组魍浆濂球燮融B浚述F调,垮畿融腰组擎科巍终袋⋯欢。继论:Z24怒太凝矮有豺魅褥矬{融裂H爵球燮国8和ASS罐佟
3、为Z24瓣蹲饿的潜巍燕物桥漤物;诱导犴缀翘澜东磷缝怒Z24翳辫矬的童器佟明樵黝之一。荚键词:灞壤攀;姐紫瀵翻蕊爨l;戳向魄泳;轩澎慑;Z24ProteomicsanalysisofplasmafromratsadministratedorallywithZ24WANGYing豫1,试魄NGQuanjunI,DongYanshen91,YuanBenlilMAHuazhi’,wUChunqi1,LIAOMingyan91弛InstituteofPharmacologyandToxicology,AcademyofMilitaryMedic
4、alSciences,NationalBeijhlgCenterforDrugSafetyEvaluationandResearch,Beijing10085敝China;2,!nstituteofMateriaMedica,LiaoningMedicalColleg鼙,Jinzhou121002,LiaoningProvince,China)Abstract:Objective:ToseekpotentialbiomarkersandmechanismofZ24·inducedhepatotoxicitybyplasmaproteom
5、icsanalysis,Methods:Wistarratswereadministratedorallywith0,50,100and200mg&gZ24for5daysrespectively。Followedtreatmentplasmabiochemistryandliverhistopathoiogyweredetected.andplasmaproteinwe豫analyzedbytwo—dimensionaIgelelectrophoresisandmassspectrometry(2*DE·MS),thenplasmaf
6、etubproein(fetuin-B)werecheckedbyWesternblot,Results:Plasmaaspav,ateaminotran_sferase,alkalinephosphatase,totalbilirubin,triglycerideandtotalcholesterol(Tch)increasedandbloodllreanitrogen(SUN)decreasedin100and200mg/kggroups。像素镯舟:壬蘩,窘.博”}?,讲师,熏婺扶攀药物镶壤掌研兜。Email:wangyin9610
7、8@163。coan。源讯髂囊:怨溺潮,粥,骄究受,鼹‘套象弩卿,熏饕从誉熬物诲蘧譬骈絮;E-mail:liaomy@nic。bmi。ac,cIl。lIncreasedTchanddecreasedBUNalsowasfoundin5铀1∥螅group。Hepatocytesvacuolardegenerationanddesmoplasiawereobservedinall7_24一treatedgroups,justatdifferentextent,bylightmicroscope。Proteomicanalysisshowed
8、theexpressionofplasmafetuin·Bdecreasedandargininosuccinatesynthase,haptoglobin,apolipoproteinEprecursor
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