后肢悬吊后大鼠比目鱼肌的蛋白质组学解析

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1、万方数据第27卷第4期深圳大学学报理工版V01．27No．42010年】0月JOURNALOFSHENZHENUNWERSITYSCIENCEANDENGINEERINGOct．2010Abstract：1000-2618(2010)04—0502—03【LifeScience】·ResearchBriefing·●_～-一ProteomicsanalysisonratssoleusmusclefibersfollowinghindlimbsuspensionWANGXiao·dong，JIANGYong，andCHENMin-shengNormal

2、CollegeShenzhenUniversityShenzhen518060P．R．ChinaAbstract：Effectsof16daysofhindlimbsuspensiononthecharacteristicsofsinglesoleusmusclefiberwereeom—paredbetweenmaleWistarandWistarlr-Iannoverrats．ThegreatereffectsofunloadingwerenotedinWistarHanno．verrats．Theunloading-relatedreducti

3、onsofmuscleweightandfibercross·sectionalareaversusthepre-suspensionlevelsweregreaterthanWistarrats．TotalnumberofmyonucleiincontrolsituationwasgreaterinWistarHannoverrats，butthemorenumbersofmyonucleiweredecreasedfollowingunloading．Responsesofmyonucleardomainlevelsweresimilar．’rh

4、enumbersofbothquiescentandmitoticactivesatellitecellsincontrolsituationweregreaterinWistarrats．Butthemagnitudeoftheunloading—relateddecreasewasidenticalforWistarHannoverandWistarrats．Althoughthelevelofheatshockprotein27(HSP27)expressioninWistarratswasdecreasedbyunloading，denovo

5、appearanceofHSP27wasnotedinWistarHannoverrats．ItissuggestedthatgreaterresponsesofsoleusmusclefibersofWistarHannoverthanWistarratsmayberelatedtothedifferentexpressionofprotein，althoughtheprecisemechanismisstillunclear．Keywords：proteomics；soleusmuscle；muscleatrophy；hindlimbsuspen

6、sion；differentialexpressedproteinCLCnumber：R337；R852．22Documentcode：ARatsolensmuscle．whichisknownasaslow-twitchanti-gravitymuscle，isontogeneticallydevelopedatl-Genviron-ment【1引．Spaceflightanditssimulationmodels．suchashind—limbsuspensionandbedrest，inducemuscleatrophyandshiftoffi

7、berstowardfast．twitchtype【4剖．However．themechanismsresponsibleforsuchphenomenaarenotclear．Thepresentstudywascarriedouttoinvestigatethedifferentresponsesofthecharac·teristicsofsinglesoleu8musclefiberinWistarandWistarHanno-verratsto16daysofhindlimbsuspension．MethodsFivcweeksoldmal

8、eWistarandWistarHannoyerratswereseparatedrandomlyintop