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1、中国水稻科学(ChinJRiceSci),２０１６,３０(３):３０４－３１２http://www．ricesci．cn３０４DOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１５０粳稻超亲变异系籽粒谷氨酰胺合成酶基因表达特性及序列变异分析徐振华１,２曲莹１刘海英１,２朱立楠１张忠臣１金正勋１,*(１东北农业大学农学院,哈尔滨１５００３０;２黑龙江省农业科学院五常水稻研究所,黑龙江五常１５０２２９;∗通讯联系人,EＧmail:zxjin３２６＠hotmail．com)ExpressionCharacteristicsandSequenceVariationAnalysisofG
2、lutamineSynthetaseGeneinGrainofjaponicaRicewithTransgressiveVariationXUZhenＧhua１,２,QUYing１,LIUHaiＧying１,２,ZHULiＧnan１,ZHANGZhongＧchen１,JINZhengＧxun１,*(１NortheastAgriculturalUniversity,Harbin１５００３０,China;２WuchangRiceResearchInstitute,HeilongjiangAcademyofAgriculturalSciences,Wuchang１５０２２９,China;∗Cor
3、respondingauthor,EＧmail:zxjin３２６＠hotmailc．om)XUZhenhua,QUYing,LIUHaiying,etal．Expressioncharacteristicsandsequencevariationanalysisofglutaminesynthetasegeneingrainofjaponicaricewithtransgressivevariation．ChinJRiceSci,２０１６,３０(３):３０４Ｇ３１２．Abstract:Thetransgressivevariantsderivedfromricevarietieswiths
4、ignificantdifferenceingrainproteincontentwereusedtoinvestigatetheexpressionlevelandstructureofglutaminesynthetasegene(GS),activityofglutaminesynthetaseandproteinaccumulationcharacteristicsduringgrainfilling．Theresultsshowedthattransgressivevariantscouldbeobtainedthroughsuccessivedirectiveselection
5、ofgrainproteincontent,becausethegrainproteincontentwascloselyrelatedtocorrespondinggenotype．Duringgrainfilling,theactivityofglutaminesynthetasefollowedasingleＧpeakcurve,highlycorrelatedwithgrainproteincontentandsignificantlydifferedbetweenparentsandprogenies．GS１．３andGS２genes,intransgressivevariant
6、sandtheirparentswithdifferentproteincontent,exhibitedsimilartranscriptiontrendduringgrainfilling,thatis,thetranscriptionlevelincreased１５－２０daysbeforeheadingandthendecreasedgradually．Moreover,thegrainproteincontentwascloselyrelatedtoGS１．３andGS２expressionlevels．ThevarietieswithhigherGStranscriptionl
7、evelshowedhigherproteincontentcomparedwiththeirparents．Inaddition,althoughGS１．３andGS２genesequencesshowedhighconservation,thegenesequenceandproteinsequenceofGS１．３andGS２werenotcompletelyidenticalindifferentvarietie
