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1、热带作物学报2014,35(5):992-998ChineseJournalofTropicalCrops千层金精油有机溶剂提取工艺的优化及成分分析叶征美1,21,21,21,2,刘鑫,沈明娟,陈建烟陈晓丹1,21,2*1,2*,李永裕,吴少华1福建农林大学园艺学院,福建福州3500022福建农林大学园艺植物天然产物研究所,福建福州350002摘要采用有机溶剂萃取法提取千层金精油,在单因素试验的基础上,以石油醚(60~90℃)为提取溶剂,选取提取时间、提取温度、料液比为影响因素,用正交试验优化千层金精油的提取工艺,并采用气相色谱-质谱(GC-MS)技术鉴定了千层金精油的化学成分。结果表明,千层
2、金精油的最佳提取工艺条件为:提取时间3h,提取温度50℃,料液比1∶20,在此工艺条件下的精油提取得率4.51%。GC-MS分析结果表明,千层金精油共含有41个组分,主要成分为甲基丁香酚,相对含量达到了83.55%,其次为肉桂酸甲酯(4.55%)、3,4,5-三甲氧基苯甲酸甲酯(1.81%)、大根香叶烯D(1.36%)。关键词千层金;精油;有机溶剂提取;正交试验;气相色谱-质谱中图分类号TQ65文献标识码AOptimizationofProcessforExtractionfromMelaleucabracteataEssentialOilwithOrganicSolventandChemic
3、alCompositionIdentify1,21,21,21,2YEZhengmei,LIUXin,SHENMingjuan,CHENJianyan1,21,2*1,2*CHENXiaodan,LIYongyu,WUShaohua1CollegeofHorticulture,FujianAgriculturalandForestryUniversity,Fuzhou,Fujian350002,China2InstituteofHorticulturalPlantsNaturalProducts,FujianAgricultureandForestryUniversity,Fuzhou,Fuj
4、ian350002,ChinaAbstractTooptimizetheextractionprocessofMelaleucabracteataessentialoilbyorganicsolvent.Usingpetroleumether(boilingpoint60-90℃)astheextractingsolvent.Theeffectsofextractiontime,extractiontemperatureandmaterial-liquidratioontheextractionM.bracteataoilwereselectedfororthogonaldesigntoopt
5、imizetheextractionprocessbasedonsinglefactortests.Gaschromatography-massspectrometry(GC-MS)technicalwasusedtoidentifythechemicalcompositionofM.bracteataessentialoil.Theresultsindicatedthattheoptimumextractionconditionwasthat:extractiontime3h,extractiontemperature50℃,material-liquidratio1∶20.Thechemi
6、calcomponentswereanalyzedbyGC-MS,and41peakswereseparatedandidentified.ThetestresultsshowedthatthemaincomponentoftheessentialoilofM.bracteataleaveswasmethyleugenol,anditsrelativecontentwas83.55%,followedbymethylcinnamate(4.55%),benzoicacid,3,4,5-trimethoxy-,methylester(1.81%),andgermacreneD(1.36%).Ke
7、ywordsMelaleucabracteata;Essentialoil;Extractionbyorganicsolvent;Orthogonaldesign;GC-MSdoi10.3969/j.issn.1000-2561.2014.05.027千层金(Melaleucabracteata),又名黄金宝树、世界上最流行、视觉效果最好的色叶乔木新树种之黄金香柳,属于桃金娘科白千层属,为常绿乔木