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1、ISSN0976–3333AvailableOnlineatwww.ijpba.info.InternationalJournalofPharmaceutical&BiologicalArchives2010;1(3):291-299ORIGINALRESEARCHARTICLEInVitroAntibacterialActivityandPhytochemicalAnalysisofSomeSelectedMedicinalPlants1121PathmanathanM.K,UthayarasaK,JeyadevanJ.PJey
2、aseelanE.C*,1DepartmentofBotany,FacultyofScience,UniversityofJaffna,SriLanka.2DepartmentofChemistry,FacultyofScience,UniversityofJaffna,SriLanka.Received27June2010;Accepted9Aug2010ABSTRACTMedicinalplantsareextensivelyusedintraditionalmedicinetocurevariousinfectiousdis
3、easesinhuman.Thepresentstudywasundertakentoinvestigateinvitroantibacterialactivityofsuccessivelyextractedhexane,dichloromethane(DCM),ethylacetate,ethanol,methanolandaqueousextractsofbulbofAlliumsativum,leafofEucalyptuscitriodoraandOcimumsanctumagainstBacillussubtilis,
4、Staphylococcusaureus,PseudomonasaeruginosaandEscherichiacoliatdifferenttestconcentrationbyagarwelldiffusionmethod.Thetestsampleswerealsosubjectedtodetectthepresenceofphytochemicals.Theresultsofantibacterialactivitywasanalyzedbyusingone-wayanalysisofvariance(ANOVA)andf
5、ollowedbyLeastSignificantDifference(LSD)test.Thestudyrevealedthattheextractspossessedantibacterialactivityinadosedependentmanner.AmongthetestplantsE.citriodorashowedbetteractivityagainsttestbacteria.AqueousextractofE.citriodoraexhibitedsignificantly(P<0.05)highereffec
6、tonB.subtilisattheconcentrationof0.5mg/100µl.MethanolandaqueousextractsofE.citriodoraonB.subtilis,ethylacetateandethanolextractofE.citriodoraonS.aureusandmethanolextractofE.citriodoraonP.aeruginosashowedsignificantly(P<0.05)highereffectattheconcentrationof1.0mg/100µlc
7、omparedtoothertestextracts.AmongthedifferenttestsamplesofO.sanctum,ethanolextractproducedbetterinhibitiononB.subtilis.EthylacetateextractofA.sativumandaqueousextractofO.sanctumshowedinhibitoryeffectonalltestbacteriaattheconcentrationrangedfrom1.0mg/100µlto30.0mg/100µl
8、.Phytochemicalstudyrevealedthattannins,alkaloids,cardiacglycosides,saponinsandterpenoidsarepresentinethylacetateandethanolex
