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1、广林九号桜叶精油的抑菌活性研究作者：m玉红周琪颇俊华孟陆丽李梓來源：《湖北农业科学》2013年笫21期摘要:研究了丿林九号^［EucalyptusurophyllaxEucalyptusgrandis(Guanglin9)］叶精油的抑菌活性。利用气相色谱■质谱联用技术分析桜叶精油的化学成分，采用滤纸片法评价其抑菌活性，以平板稀释法测定了粘:油的最小抑菌浓度。广林九号桜叶粘油的主要成分有：1,8■桜叶油素(40.844%)、a■兼烯(24.221%)、a■松油醇(4.661%)、反式•松香芹醇(3.735%)、龙脑(2.762%
2、)、乙酸松油脂(3.592%)和松香芹酮(1.978%)。广林九号桜叶精油对测试的菌种均有良好的抑菌活性，但对细菌和霉菌的抑菌效果有所差异。对金黄色葡萄球菌(Staphylococusaureus)、枯草芽抱杆菌(Bacillussubtilis)、大肠杆菌(Escherichiacoli),黄曲霉(Aspergillusflavus)、黑曲霉(Aspergillusniger)和橘青霉(Penicillumcitrinum)的最小抑菌浓度分别为10、20、20、10、10、5mL/L。关键词：广林九号桜［Eucalyptus
3、UrophyllaxEucalyptusGrandis(Guanglin9)］叶；精油;抑菌活性；最小抑菌浓度中图分类号:TQ654+.2文献标识码：A文章编号:0439-8114(2013)21-5193-03StudiesonAntimicrobialActivityofEssentialOilfromLeavesofEucalyptusurophy11axEucalyptusgrandis(Guanglin9)TIANYu-hongl,ZHOUQ订，POJun-hua2,MENGLu-lil,LIZ订(1・Departm
4、entofBiologicalandChemicalEngineering,GuangxiUniversityofTechnology,Liuzhou545006,Guangxi,China；2.LiuzhouHualiHouseholdProductsLimitedCompany,Liuzhou545006,Guangxi,China)Abstract：TheantimicrobialactivityoftheessentialoilfromleavesofEucalyptusurophyllaxEucalyptusgran
5、dis(Guanglin9)wasstudied.Theessentialoilwasanalyzedbygaschromatography-massspectrometry(GC-MS)method.Theantimicrobialactivityofoilwasevaluatedbyusingfilterpapermethodandtheminimuminhibitoryconcentration(MIC)wasdeterminedbyplatedilutionmethod・Themaincomponentsofthees
6、senti-aloilwere1,8-cineole(40.844%),followedbya-pinene(24.221%),a-terpineol(4.661%),a-terpinylacetate(3.592%),borneol(2.762%),trans-pinocarveol(3.735%)andpinocarvone(1.978%).Theresultsindicatedthattheessentialoilhadexcellentantimicrobialactivityagainsttestedmicroorg
7、anisms.Theeffectsofessentialoilonantibacterialandantifiingalactivitiesdifferedobviously.Theminimuminhibitoryconcentrations(MICs)were10,20,20,10,10and5mL/LforStaphylococusaureus,Bacillussubtilis,Escherichiacoli,Aspergillusflavus,AspergillusnigerandPenicillumcitrinum,
8、respectively.Keywords：EucalyptusurophyllaxEucalyptusgrandis(Guanglin9)；essentialoil；antimicrobialactivity；minimuminhibitoryconcentration广讥
