biochemistry and biotechnology of amino acid

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1、BiochemistryandBiotechnologyofAminoAcidDehydrogenasesToshihisaOhshima1andKenjiSoda2DepartmentofChemistry,KyotoUniversityofEducation,Fushimi-ku,Kyoto612,Japan2LaboratoryofMicrobialBiochemistry,InstituteforChemicalResearch,KyotoUniversity,Uji,Kyoto-Fu611,Japan1Introduction.

2、.................................................................1882MolecularandCatalyticPropertiesofAminoAcidDehydrogenases..................1892.1GlutamateDehydrogenases...................................................1892.2AlanineDehydrogenase.......................

3、..............................1912.3LeucineDehydrogenase.....................................................1952.4PhenylalanineDehydrogenase...............................................1952.5OtherAminoAcidDehydrogenases...........................................1973Ste

4、reospecificityofHydrogenTransferofCoenzymeandKineticMechanismofAminoAcidDehydrogenaseReaction...................................................1974GeneCloningandPrimaryStructureofAminoAcidDehydrogenases.................2005ApplicationofAminoAcidDehydrogenases............

5、...........................2025.1ProductionofL-andD-AminoAcids..........................................2025.2PreparationofStereoselectivelyDeuteratedNADHandNADPH.................2035.3ApplicationsofAminoAcidDehydrogenasestoSpecificQuantitativeAnalyses.....2056References.

6、...................................................................206Overthelastdecade,aminoaciddehydrogenasessuchasalaninedehydrogenase(AlaDH),leucinedehydrogenase(LeuDH),andphenylalaninedehydrogenase(PheDH)havebeenap-pliedtotheenantiomer-specificsynthesisandanalysisofv

7、ariousaminoacids.Inperticu-lar,aminoaciddehydrogenasesfromthermophileshavereceivedmuchattentionbecauseoftheirhighstability.Theirproductivitywasenhancedandthepurificationfacilitatedbythegenecloning.Theadvancesinbiotechnologicalapplicationsoftheseenzymesarebasedonfunda-ment

8、alstudiesconcerningcharacteristicsoftheenzymesandreactionmechanismasdescribedinthischapter.Furth