amino+acid+composition+and+functional+properties+of+giant+red+sea+cucumber+（parasfichopus+californicus）+collagen+hydrolysates

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1、OceanUniv．China(OceanicandCoastalSeaResearch1D0I10．1007／s11802．0l1一l7874ISSN1672—5182，20I110(1)：80—84http：／／www．ORe．edu．cn／xbywb／E—mail．"xbywb@OblC．edu．cnAminoAcidCompositionandFunctionalPropertiesofGiantRedSeaCucumber(Parastichopuscalifomicus)CollagenHydrolysatesLIUZunying¨SUYicheng，andZENG

2、Mingyong)，，1、College吖FoodScienceandEngineering,OceanUniversityDfChinaQingdao266003PRChina2)SeaJoodResearchandEducationCenter，OregonStateUniversity，2001MarineDrive，Room253，Astoria，OR97103，US．4(ReceivedMay10，2010；revisedJuly15，201O：acceptedJuly19，2010)@OceanUniversityofChina．SciencePressandSpr

3、ing—VerlagBerlinHeidelberg2010AbstractGiantredseacucumber(Parastichopuscalifornicus)isanunder—utilizedspeciesduetoitshightendencytoautolysis，Iheaimofthisstudywastoevaluatethefunctionalpropertiesofcollagenhydrolysatesfrointhisspecies．Thedegreeofhydrolysis(DH)，aminoacidcomposition，SDS—PAGE，emu

4、lsionactivityindex(EAI)，emulsionstabilityindex(ES1)，foamexpansion(FE)．andfoamstability(FS)ofhydrolysateswereinvestigated．TheefectsofpHontheEAI，ESIFEandFSofhydrolysateswerea1soinves．tigated．Theresultslndicatedthatthe∥andalchainsofthecollagenwcrcefectivelyhydrolyzedbytrypsinat50℃withanEn．zyme／

5、Substrate(E／S)rationof1：20(w：w)．TheDHofcollagenwasupto17，3％after3hhydrolysiswithtrypsinThehydrolysateshadamolecularweightdistributionof1．1—17kDa，andwereabundantinglycine(Gly)，proline(Pro)，glutamicacid(Glu)．alanine(Ala)andhydroxyproline(Hyp)residues．Thehydrolysateswerefractionatedintothreefra

6、ctions(<3kDa3-10kDa，and>10kDa1andthefractionof3-10kDaexhibitedahigherEA1valuethanthefractionof>10kDa(P<0．05)．Thefractionof>10kDahadhigherFEandFSvaluesthanotherfractions(户<0．05)．ThepHhadanimportantefectoiltheEAI，ESI，FEandFS．AllthefractionsshowedundesirableemulsionandformingpropertiesatpH4．0．U

7、nderpH7，0andpH10．0．the3—10kDafractionshowedhigherEAIvalueandthefractionof>10kDashowedhigherFEvalue，respectively．Theyarehopedtobeutilizedasfunctionalingredientsinfoodandnutraceutica1jndustries．Keywordsgiantredseacucumber；collagen；hydrolysates；functi