genetic diversity of greek aegilops species using different types of nuclear

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1、MolecularPhylogeneticsandEvolution56(2010)951–961ContentslistsavailableatScienceDirectMolecularPhylogeneticsandEvolutionjournalhomepage:www.elsevier.com/locate/ympevGeneticdiversityofGreekAegilopsspeciesusingdifferenttypesofnucleargenomemarkersKonstantinosG.Thomas,PenelopeJ.Bebeli*DepartmentofPlan

2、tBreedingandBiometry,AgriculturalUniversityofAthens,IeraOdos75,Athens11855,GreecearticleinfoabstractArticlehistory:RandomAmplifiedPolymorphicDNA(RAPD)andInter-SimpleSequenceRepeat(ISSR)analyseswereReceived7December2009usedtoevaluategeneticvariabilityandrelationshipsofGreekAegilopsspecies.Thirty-eig

3、htaccessionsRevised26April2010ofsevenGreekAegilopsspecies[Ae.triuncialis(genomeUC),Ae.neglecta(UM),Ae.biuncialis(UM),Ae.cau-Accepted29April2010data(C),Ae.comosa(M),Ae.geniculata(MU)andAe.umbellulata(U)]aswellasTriticumaccessionswereAvailableonline5May2010studied.NineteenRAPDandtenISSRprimersyielde

4、d344and170polymorphicbands,respectively,thatwereusedfortheconstructionofdendrograms.RegardlessofthesimilaritycoefficientandmarkertypeKeywords:used,UPGMAplaced38AegilopsaccessionsintoonebranchwhiletheotherbranchconsistedofwheatAegilopsspecies.WithintheAegilopscluster,subgroupswereidentifiedthatinclud

5、edspeciesthatsharedthesameRAPDISSRgenomeorbelongedtothesamebotanicalsection.WithintheTriticumcluster,tworobustsubgroupsGeneticsimilaritycoefficientswereformed,oneincludingdiploidwheatandanotherincludingpolyploidwheat.Inconclusion,resultsshowedthatthereisgeneticdiversityintheGreekAegilopsspeciesstud

6、ied,andclusteringbasedongeneticsimilaritieswasinagreementwithbotanicalclassifications.Ó2010ElsevierInc.Allrightsreserved.1.Introduction2008);Ae.geniculataisresistanttopowderymildew(StoilovaandSpetsov,2006)andBYDV(BarleyYellowDwarfVirus)(Zahari-ThegenusAegilopsbelongstofamilyPoaceaeandconsistsof22ev

7、aetal.,2001b);Ae.variabilisisresistanttonematodeHeteroderaspeciesofwhich10arediploid,10tetraploidand2hexaploidwithavenae(Barloyetal.,2007).Aegilopsspeciescanalsocontributetobasicchromosomenumberx=n=7(vanSlageren,