genetic diversity of mango cultivars estimated using scot and issr

genetic diversity of mango cultivars estimated using scot and issr

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1、BiochemicalSystematicsandEcology39(2011)676–684ContentslistsavailableatScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversityofmangocultivarsestimatedusingSCoTandISSRmarkersaa,b,*aaacCongLuo,Xin-huaHe,HuC

2、hen,Shi-jinOu,Mei-pingGao,JamesStevenBrown,ccCecileT.Tondo,RaymondJ.SchnellaCollegeofAgriculture,GuangxiUniversity,Nanning530004,PRChinabGuangxiCropGeneticImprovementandBiotechnologyLab,Nanning530007,PRChinacNationalGermplasmRepository,USDAARSSHRS,Miami,USAarticle

3、infoabstractArticlehistory:Twomolecularmarkersystems,SCoTandISSRwereusedforidentificationandgeneticReceived28December2010comparisonanalysisof23mangogermplasmaccessionscollectedwithinGuangxiprov-Accepted28May2011inceofChina.Using18selectedSCoTprimers158bandsweregene

4、rated,ofwhich104Availableonline16June2011(65.82%)werepolymorphic.EighteenselectedISSRprimersamplified156bandswith87(55.77%)beingpolymorphic.ThecultivarsofXiangYaMangotypeandtheirprogenyhaveKeywords:highgeneticsimilaritywitheachother.The23cultivarswereclusteredintot

5、womajorSCoTmarkersgroupsbasedontheSCoTanalysisandthreemajorgroupsbasedontheISSRanalysiswithISSRUPGMA.TheseclustersareinaccordancewiththeirknownoriginsandmainphenotypicGeneticdiversityMangiferaindicaL.characteristics.OurresultsindicatedthattheSCoTanalysisbetterrepr

6、esentstheactualrelationshipsthanISSRanalysis,althoughbothanalysesgivesimilarresults.TheresultsalsodemonstratethattheSCoTmarkersystemisusefulforidentificationandgeneticdiversityanalysisofmangocultivars.Ó2011ElsevierLtd.Allrightsreserved.1.IntroductionMango(Mangifera

7、indicaL.)isanimportantmemberofthefamilyAnacardiaceaeandisanimportantfruittreecropgrowncommerciallyintropicalandsubtropicalregionsoftheworld(Rajwanaetal.,2011).Mangogenotypesaredividedintotwodistinctcategories:monoembryonicmangos,whicharemostlysubtropical(Indiantyp

8、es)andpolyembryonicmangos,whicharemostlytropical(SoutheastAsiantypes).TheseedsofIndiantypescharacteristicallycontainazygoteembryoandthefruitskinishighly

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