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1、BiochemicalSystematicsandEcology45(2012)209–217ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticdiversityanalysisofPrunellavulgarisinChinausingISSRandSRAPmarkersa,ba,*baaLiLiao,Qia
2、o-shengGuo,Zhi-yongWang,LiLiu,Zai-biaoZhuaInstituteofChineseMedicinalMaterials,NanjingAgriculturalUniversity,Nanjing,Jiangsu210059,PRChinabAgronomyCollege,HainanUniversity,Danzhou,Hainan571737,PRChinaarticleinfoabstractArticlehistory:Thisstudyassessedthemoleculargenet
3、icdiversityandrelationshipsamong25wildReceived4March2012PrunellavulgarisL.populationsandonecultivatedpopulationinChina.TheresultsshowedAccepted22July2012thatallthe26P.vulgarispopulationspossessedhighlevelsofgeneticdiversity.However,Availableonline6September2012polymor
4、phismwasrelativelylowerinthecultivatedpopulation.SeventeenSRAPprimercombinationsgenerated153amplificationbands,ofwhich134werepolymorphic.Keywords:However,195polymorphicbandsweredetectedoutof219bandsthatwereamplifiedPrunellavulgarisL.using20ISSRprimers.ISSRmarkersshoweda
5、slightlyhigherproportionofpolymorphicGeneticdiversityloci(PPL¼89.19%)thandidtheSRAPmarkers(PPL¼87.93%).TheresultsalsoshowedthatISSRmarkersSRAPmarkersthe26populationscouldbeclusteredintothreegroupsbyclusteranalysis,whichmatchedthegeographicdistributionofthisspecies.The
6、dataindicatedthatISSRandSRAParebothreliableandeffectivetoolsforanalyzinggeneticdiversityinP.vulgaris.Ó2012ElsevierLtd.Allrightsreserved.1.IntroductionPrunellavulgaris,knownas“self-heal”or“heal-all”isalonglivedmedicinalherbwithaworldwidedistribution.Ithasbeenusedasatra
7、ditionalremedyinEuropeandChinaforthousandsofyears(Pinkasetal.,1994;Kimetal.,2007).ThefruitspikesofP.vulgarishavebeenusedtotreatsorethroat,feverandwoundsandthereisanincreasingcommercialdemandforthisplantduetoitspotentialanti-inflammatory,antiviralandantibacterialactivit
8、y(Chiuetal.,2004;Kageygamaetal.,2000;Psotováetal.,2003;Zhangetal.,2007).WildP.vulgarishasbeenfoundgrowingalongthenorthernand
