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1、Heredity(2008)101,136–144&2008NaturePublishingGroupAllrightsreserved0018-067X/08$30.00www.nature.com/hdyORIGINALARTICLEAnintegratedapproachtothecharacterizationoftwoautochthonouslentil(Lensculinaris)landracesofMolise(south-centralItaly)GSScippa1,DTrupiano1,MRocco2,VViscosi1,MDiMi
2、chele3,ADAndrea1andDChiatante41DipartimentodiScienzeeTecnologieperl’AmbienteeilTerritorio,UniversityofMolise,Pesche,Italy;2DipartimentodiScienzeBiologicheeAmbientali,UniversityofSannio,Benevento,Italy;3LaboratoryofAnalyticalTechniquesandProteomics,‘JohnPaulII’CenterforHighTechnol
3、ogy,ResearchandEducationinBiomedicalSciences,CatholicUniversity,Campobasso,Italyand4DipartimentodiScienzeChimicheedAmbientali,UniversityofInsubria,Como,ItalyPlantbiodiversitymustbesafeguardedbecauseitconstitu-intheMolisegermoplasmbank.Thetwolocallandracestesaresourceofgenesthatma
4、ybeused,forinstance,inwerewelldifferentiatedfromeachother,andtheConcabreedingprograms.Lentil(LensculinarisMedik.)isoneofCasalelandracewasseparatedfromthecommercialthemostancientcropsoftheMediterraneanregion.varietiesatmorphological,proteinandDNAlevel.TheExtensivedifferentiationof
5、L.culinarisovermillenniahasCapracottalandrace,waswellseparatedfromthecommer-resultedinamyriadofdifferentlandraces.However,inmorecialvarieties,exceptCastellucciodiNorcia,atDNAlevelrecenttimesmanylandraceshavedisappearedconsequentshowingamorecomplexandheterogeneoussegregationtoenvi
6、ronmentalandsocioeconomicchanges.Topromoteatmorphologicalandbiochemicallevel.Thecorrelationthesurvivalofendangeredlentillandraces,wehavebetweenmorphological,DNAandproteindata,illustratesinvestigatedthegeneticrelationshipbetweentwoancientthatproteomicsisapowerfultoolwithwhichtocom
7、plementlandracecultivatedinCapracottaandConcaCasale(Molise,theanalysisofbiodiversityinecotypesofasingleplantsouth-centralItaly)andwidelyspreadcommercialvarietiesspeciesandtoidentifyphysiologicaland/orenvironmentalusinganintegratedapproachconsistingofstudiesatmarkers.morphological
8、,DNAandproteinlevel.SeedsofthesetwoHered
