大黄牡丹汤含药血清对 LPS 刺激的小鼠肺巨噬细胞 TLR4和 MyD88表达的影响-论文.pdf

《大黄牡丹汤含药血清对 LPS 刺激的小鼠肺巨噬细胞 TLR4和 MyD88表达的影响-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、山东医药2014年第54卷第10期大黄牡丹汤含药血清对LPS刺激的小鼠肺巨噬细胞TLR4和MyD88表达的影响孙燕妮，承解静。杨晓燕，杨洁。王丽敏。刘军(上海中医药大学附属普陀医院，上海200062)摘要：目的探讨大黄牡丹汤含药血清(以下简称含药血清)对脓毒症的治疗机制。方法体外培养小鼠肺巨噬细胞株RAW264．7并随机分为空白组，模型组，含药血清高、中、低浓度组，每组3个复孔。除空白组外，其余四组均予细菌脂多糖(LPS)5g／mL刺激24h，含药血清高、中、低浓度组分别同时给予高、中、低浓度的含药血

2、清进行干预。干预后于37℃、5％CO：培养箱中培养24h。分别采用半定量RT—PCR和Westernblot法测定Toll样受体4(TLR4)及髓样分化因子88(MyD88)mRNA和蛋白表达。结果模型组TLR4和MyD88mRNA和蛋白表达均显著高于空白组(P

3、分化因子88doi：10．3969／j．issn．1002—266X．2014．10．004中图分类号：R563．9文献标志码：A文章编号：1002-266X(2014)10-0010-04EfectofDahuangMudandecoction-containedserumontheexpressionofTLR4andMyD88inlipopolysaccharide-stimulatedpulmonarymacrophagesofratsSUNYan-ni，CHENGJie4ing，YANGXi

4、ao-yan，YANGJie，Li—min，LIUJun(PutuoHospitalAffiliatedtoShanghaiUniversityofTCM，Shanghai200062，China)Abstract：0bjectiveToinvestigatethetherapeuticmechanismofDahuangMudandecoction—containedserum(here-inafterreferredtoasthedrugcontainedserum)inthetreatmento

5、fsepsis．MethodsMousepulmonarymacrophagecelllineRAW264．7wasculturedinvitro，andthenthecellswererandomlydividedintotheblankgroup，modelgroup，high-，moderate-andlow-concentrationdrug·containedselumgroups，eachgroupof3holes．Excepttheblankgroup，otherfourgroupswe

6、restimulatedwithbacteriallipopolysaccharide(LPS)5g／mL，thenweretreatedwithdifferentconcentrationsofdrug-containedserumfor24h．Aftertheintervention，thecellswereculturedin5％CO2at37oCfor24h．ThenthemR—NAexpressionofToll-likereceptor4(TLR4)andmyeloiddifferenti

7、ationfactor88(MyD88)wasmeasuredbysemi-quanti—tativereversetranscription—polymerasechainreaction(RT-PCR)，andtheirproteinexpressionwasanalyzedbyWesternblotting．RemitsTheproteinandmRNAexpressionofTLR4andMyD88inthemodelgroupwassignificantlyhigherthanthatoft

8、heblankgroup(P<0．01)，buttheproteinandmRNAexpressionofTLR4andMyD88inthehigh—andmoderate—concentrationdrug—containedserumgroupswassignificantlylowerthanthatofthemodelgroup(P<0．o1)，andnosignifi—cantdifferenceswasfoundbetweenthehigh—