整合素αvβ3受体靶向载药脂质体的制备及体外靶向性.pdf

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1、中国组织工程研究与临床康复磐74眷劳47-2010—11—19出版JournalofClinicalRehabilitative~ssueEngmeefingResearchNovember19，2010Vo1．14，No．47整合素aVI33受体靶向载药脂质体的制备及体外靶向性术★解旭品，周兆熊，胡亚立，张柏根，张纪蔚Preparationofintegrinav133targetedliposomeanditstargetedabilityinvitroXieXu‘pin，ZhouZhao-xiong，H

2、uYa—li，ZhangBai-gen，ZhangJi-weiAbstractDepartmentofBACKGROUND：RGD—containingpeptidesarerecognitionsitesofavarietyofintegrinandarewidelyusedinthedesignofVascularSur口etargetingnanoparticlesfordrugdeliverysystemduetoitssmallmolecularweightRenjiHospita1．．stabil

3、ity，easypreparation，andnoShanghaiJiao1bn0immunogenicity．UniversitySchooIofOBJECTIVE：Tosynthesizetheintegrinav~B3targetedliposome，loadingdrugsandwithRGDoligopeptideasmainbindingMedicine。Shanghajcomponents，andtoassessItsreceptortargetedabilitythrough『门vitrocy

4、tologyexperiment．200001，ChinaMETHODS：ThesyntheticRGDoligopeptideservedastargetedmolecularprobe，theliposomesconjugatedwithArg．Gly-AspXieXu—pin★．(RGD)peptidesweremadebyhighpressurehomogeneousprocessingmethods．ThemorphologyandsizewereexaminedbyStudyingformaste

5、r’sscanningelectronmicroscopeandIaserparticlesizeanalyzer．Thespecificmarkofvascularsmoothmusclecelswasanalyzeddegree．DepartmentofVascularSurgery,withflowcytometry，aswelIastheexvivoreleaseand『门vitrotargetingabilityofloadeddrugs．RenjiHospita1．RESULTSANDCONCLU

6、SION：Theparticlesizeoftargetedliposomewas(175+6)nm，andtheencapsulationeficiencywasShanghaiJiaoT0naUniversitySchooIof(9633+102)％Thetargetedliposomeloadingdrugscouldreleasepersistedover5daysinvitroThetargetedliposomehadMedicine．Shanghaispecificafinityforav133

7、integrinandcouldentercellswithrecept0卜mediatedendocytosis．TheintegrinavB3targetedliposomes200001．Chinahaverelativelyhighencapsulationeff~ciencyandsustainedreleasepattern，itcanbindparticularlywithav133integrinandmayxiexupin@163combecomeanewtypereceDt0卜mediat

8、edtargetingagentCorrespondenceto：ZhangJi-wei．XieXP，ZhouZX．HuYL，ZhangBG，ZhangJWPreparationofintegrinave3targetedIiposomeanditstargetedabilityinvfroProfessor,Chiefphysician．ZhongguoZuzhiGongchengYanjiuyu