灯盏花素调控MICA基因表达对人肾小管上皮细胞缺氧再给氧损伤的保护作用.pdf

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1、免疫学杂志2014年8月第30卷第8期IMMUN0L0GICALJOURNALVo1．30No．8Aug．2014·691··论著·【文章~]1000-8861(2014)08-0691-04；[DOI]10．13431／j．enki．immuno1．j．20140151灯盏花素调控MICA基因表达对人肾小管上皮细胞缺氧再给氧损伤的保护作用罗蕾，李震宇△，罗光恒，夏曼，盛长城，胡誉怀，方茜【摘要】目的观察灯盏花素提取液对人肾小管细胞(HK一2)缺氧再给氧(hypoxia／re—oxygenation

2、，H／R)损伤的保护作用，探讨其可能的治疗作用及保护机理。方法建立HK一2体外H／R损伤模型。随机分为3组：对照组、缺氧组、灯盏花素组(灯盏花素预处理+缺氧)。在缺氧16h后设立4个时间点：复氧0、4、8、16h。倒置显微镜下观察细胞的形态学改变，荧光定量和流式检测各组HK一2MICA分子和蛋白水平的表达，乳酸脱氢酶释放法测定NK细胞对HK一2的杀伤能力。结果与对照组相比，缺氧组HK一2细胞MICA转录水平和蛋白表达水平在缺氧16h后显著升高，于复氧8h达峰值后逐渐下降，并于复氧16h后恢复正常。N

3、K细胞对H／R处理的HK一2细胞的杀伤活性与MICA的表达变化呈正相关。与缺氧组相比，灯盏花素组显著降低H／R处理的HK一2细胞MICA的表达和NK细胞对其的杀伤活性。结论在IRI过程中，下调H／R处理的HK一2细胞的MICA，从而抑制NK细胞对其的杀伤活性，可能是灯盏花素抗氧化应激和增强抗氧化防御功能的有效机制之一。[关键词】人肾小管上皮细胞；缺氧再给氧；灯盏花素；NK细胞毒实验[中图分类号】R392．5[文献标识码】A．Theprotectiveeffectsofbreviscapineonhu

4、manrenalproximaltubularepithelialcellsduringhypoxia／re-oxygenationinjuryviaregulatingMICAexpressionLUOLei，LIZhengyu，LUOGuangheng，XIAMin，SHENGChangcheng，HUYuhuai，FANGQianDivisionofScienceandEducation,People’SHospitalofGuizhouProvince，Guiyang550002，China

5、CorrespondingAuthor：FANGQian,E-mail：969824080@qq．conATheseauthorscontributedequallytothisworkandsharefirstauthorship[Abstract]Thisstudydesignedtoinvestigatetheprotectiveroleofbreviscapineonhumanrenalproximaltubularepithelialcells(HK-2)anditsrelativemec

6、hanismduringhypoxia／re-oxygenation(H／R)．Firstly，ischemia／reperfusion(I／R)injury(IRI)wasmimickedthroughestablishingaHRmodelofHK-2cellsinvitro．HK一2cellswererandomlydividedintothreegroups：control，H／Rgroupandbreviscapinetreatedgroup(breviscapinepretreatmen

7、t+H／R)．Sixteenhoursafterhypoxia，HK一2cellswerereoxygenatedfor0，4，8，16hrespectivelyundernormalcondition．QuantitativerealtimePCRandflowcytometryindicatedthatH／RsignificantlyincreasedthelevelsofMICAmRNAandproteinlevelsonHK-2，whilelactatedehydrogenase(LDH)r

8、eleaseassaydemonstratedthatH／RenhancedNKcellcytotoxicactivitytowardsHK-2．AdministrationofbreviscapinesignificantlyreducedthemRNAandproteinlevelsofMICAinI／RHK-2，andthensignificantlydecreasetheNKcellcytotoxicityactivity．Thus，weconcludedth