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时间:2020-06-16
《灯盏花素减轻顺铂对大鼠肾损害及肾细胞凋亡机制的研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、中华实用诊断与治疗杂志2014年3月第28卷第3期JournalofChinesePracticalDiagnosisandTherapy·251··论著·灯盏花素减轻顺铂对大鼠肾损害及肾细胞凋亡机制的研究马天江,张磊,张智慧,刘娜,任亮,张印坡,冯学召。(1.漯河医学高等专科学校第一附属医院肿瘤科,河南漯河462000;2.漯河医学高等专科学校基础部药理教研室,河南漯河462000)摘要:目的探讨应用灯盏花素减轻顺铂对大鼠肾损害及肾细胞凋亡的作用机制。方法48只SD大鼠分为对照组、模型组、25mg/kg灯盏花素组(灯盏花素1组)和50mg/kg灯盏花素
2、组(灯盏花素2组)各12只。对照组给予羧甲基纤维素钠溶液灌胃,模型组、灯盏花素1组和2组均腹腔注射顺铂8mg/kg造模。灯盏花素1组和2组分别以25mg/kg和50mg/kg灯盏花素灌胃,给药7d后计算4组肾脏指数,采用原位缺口末端标记法检测肾细胞凋亡情况,左肾采用蛋白质印迹法检测肾皮质Bax、Bcl一2表达水平,右肾采用免疫组织化学法检测肾组织凋亡蛋白Bax和Bcl一2表达水平。结果模型组、灯盏花素1组和2组肾脏指数、凋亡指数及Bax、Bcl一2表达水平高于对照组(P3、于模型组、Bcl-2表达水平高于模型组(P<0.05);灯盏花素1组肾脏指数和凋亡指数高于2组(P<0.05)。结论灯盏花素可增强凋亡蛋白Bcl-2表达、降低Bax表达,影响线粒体凋亡通路,从而减轻顺铂对肾损害大鼠肾细胞的凋亡。关键词:肾损害;大鼠;灯盏花素;顺铂;细胞凋亡MechanismofBreviscapineinreducingcisplatin‘inducedkidneydamageandapoptosisofkidneycellsinratsMATian-jiang,ZHANGLei,ZHANGZhi—hui,LIUNa,RENLiang,4、ZHANGYin-po,FENGXue-zhao(*DepartmentofOncology,theFirstAffiliatedHospitalofLuoheMedicalCollege,Luohe462000,China)Abstract:ObjectiveToexplorethemechanismofBreviscapineinreducingcispIatin—inducedkidneydamageandapoptosisofkidneycells.MethodsForty-eightSDratswererandomlydividedintoco5、ntrolgroup,modelgroup,25mg/kgBreviscapinegroup(Breviscapine1group)and50mg/kgBreviscapinegroup(Breviscapine2group),with12ratsineachgroup.Controlgroupwasgivenlavagewithearboxymethylcellulosesodium,andtheotherthreegroupswereestablishedmodelsbyintraperitonea1injectionof8mg/kgcisplati6、n,followedbylavagewith25mg/kgand50mg/kgBreviscapinerespectivelyinBreviscapine1and2groups.Fourdayslater,thekidneyindexeswerecalculated.TheapoptosisindexesofkidneycellsweredetectedbyTUNELmethod.TheexpressionsofBaxandBcl一2inkidneycellsweredetectedbyWesternblotmethodintheleftkidney,a7、ndtheexpressionsofBaxandBcl一2weredetectedbyimmunohistochemiealmethodintherightkidney.ResultsThekidneyindexes,apoptosisindexes,andBaxandBel一2levelswerehigherinmodelgroupandBreviscapinegroupsthanthoseincontrolgroup(P8、ower,andBcl一2levelwashigherinBreviscapin
3、于模型组、Bcl-2表达水平高于模型组(P<0.05);灯盏花素1组肾脏指数和凋亡指数高于2组(P<0.05)。结论灯盏花素可增强凋亡蛋白Bcl-2表达、降低Bax表达,影响线粒体凋亡通路,从而减轻顺铂对肾损害大鼠肾细胞的凋亡。关键词:肾损害;大鼠;灯盏花素;顺铂;细胞凋亡MechanismofBreviscapineinreducingcisplatin‘inducedkidneydamageandapoptosisofkidneycellsinratsMATian-jiang,ZHANGLei,ZHANGZhi—hui,LIUNa,RENLiang,
4、ZHANGYin-po,FENGXue-zhao(*DepartmentofOncology,theFirstAffiliatedHospitalofLuoheMedicalCollege,Luohe462000,China)Abstract:ObjectiveToexplorethemechanismofBreviscapineinreducingcispIatin—inducedkidneydamageandapoptosisofkidneycells.MethodsForty-eightSDratswererandomlydividedintoco
5、ntrolgroup,modelgroup,25mg/kgBreviscapinegroup(Breviscapine1group)and50mg/kgBreviscapinegroup(Breviscapine2group),with12ratsineachgroup.Controlgroupwasgivenlavagewithearboxymethylcellulosesodium,andtheotherthreegroupswereestablishedmodelsbyintraperitonea1injectionof8mg/kgcisplati
6、n,followedbylavagewith25mg/kgand50mg/kgBreviscapinerespectivelyinBreviscapine1and2groups.Fourdayslater,thekidneyindexeswerecalculated.TheapoptosisindexesofkidneycellsweredetectedbyTUNELmethod.TheexpressionsofBaxandBcl一2inkidneycellsweredetectedbyWesternblotmethodintheleftkidney,a
7、ndtheexpressionsofBaxandBcl一2weredetectedbyimmunohistochemiealmethodintherightkidney.ResultsThekidneyindexes,apoptosisindexes,andBaxandBel一2levelswerehigherinmodelgroupandBreviscapinegroupsthanthoseincontrolgroup(P8、ower,andBcl一2levelwashigherinBreviscapin
8、ower,andBcl一2levelwashigherinBreviscapin
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