灯盏花素对肾损害模型小鼠肾脏TGF-β_1、Col-Ⅳ表达的影响.pdf

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1、灯盏花素对肾损害模型小鼠肾脏TGF．p、Co1．IV表达的影响李向平，马菲，张印坡。，任亮。(1．济源市人民医院，河南济源454650；2．漯河市源汇区妇幼保健院，河南漯河462002；3．漯河医学高等专科学校，河南漯河462002)中图分类号R285；R96文献标志码A文章编号10010408(2013)03．0213．03DOI10．6039／j．issn．1001-0408．2013．03．07摘要目的：研究灯盏花素对肾损害模型小鼠肾功能和转化生长因子(TGF)．B、Ⅳ型胶原蛋白(Co1．IV)表达的影响。方法

2、：腹腔注射顺铂8mWkg以复制小鼠肾损害模型。实验分为对照(5％羧甲基纤维素钠)、模型(5％羧甲基纤维素钠)与灯盏花素高、低剂量(50、25mg&g)组。灌胃给药，每天1次，连续7d。给药结束后HE染色观察大鼠肾组织改变，进行肾脏指数、尿蛋白(Upr)／尿肌酐(Ucr)比值、Ⅳ-乙酰氨基葡萄糖苷酶(NAG．U)等肾功能指标测定；免疫组化法检测肾组织TGF．B。、Co1．IV的表达。结果：病理切片显示高、低剂量灯盏花素可显著改善顺铂致肾损害小鼠的肾组织结构。与模型组比较，灯盏花素高、低剂量组小鼠肾脏指数、Upr／Ucr

3、比值与NAG—U活性均显著下降(P

4、ei，ZHANGYin—po。，RENLiang。(1．ThePeople’sHospitalofJiyuanCity，HenanJiyuan454650，China；2．YuanhuiDistrictWomenandChildrenHealthCareHospitalofLuoheCity，HenanLuohe462002，China；3．LuoheMedicalCollege，HenanLuohe462002，China)ABSTRACTOBJECTIVE：Tostudytheefectofbreviscapin

5、eonrenalfunctionandtheexpressionofTGF．BlandCo1．IVinmicewithrenalimpairment．METHODS：Renalimpairmentmodelwasinducedbyintraperitonealinjectionofcisplatin(8mg／kg)．Micewererandomlydividedintocontrolgroup(5％CMC-Na)，modelgroup(5％CMC-Na)，breviscapinehigh—doseandlow—dos

6、egroups(50，25mg／kg)．Theyweregivenrelevantmedicineintragastricallyonceadayforconsecutive7days．Changesofrenaltis—suewasobservedbyHEstainingaftermedication，andrenalindex，ratioofUprtoUcr，NAG．Uandotherrenalfunctionindexweredetermined；theexpressionofTGF-BlandCo1．Ⅳinr

7、enaltissuesweredeterminedbyimmunohistochemicalmethod．RE．SULTS：Pathologicalexaminationsshowedthathigh．dose，low．dosebreviscapineimprovedtherenaltissueofmicewithcisplatin．in．ducedrenalimpairment．Comp~edwithmodelgroup，renalindex，UprAJcrratioandNAG—Uweresignificantl

8、ydown—regulatedinbreviscapinehigh．doseandlow．dosegroups(P