地塞米松对哮喘小鼠支气管肺泡灌洗液中IL-25和IFN-γ的影响.pdf

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1、医药导报2014年8月第33卷第8期·997·地塞米松对哮喘小鼠支气管肺泡灌洗液中IL一25和IFN一的影响陆韦，王蕾，谯明，王玉，江吉富，吴中明(1．遵义医学院附属医院儿科，遵义563099；2．遵义医学院微免教研室，遵义563003)摘要目的通过检测支气管肺泡灌洗液(BALF)中白细胞介素-25(IL-25)和1I一干扰素(IFN一)的水平，探讨地基米松对小鼠支气管哮喘的治疗作用机制。方法清洁级Balb／c小鼠随机分为正常对照组、哮喘组和地塞米松组。以鸡卵清蛋白(OVA)致敏激发法建立哮喘小鼠模型。地塞米松组在每次激

2、发前1h予地塞米松腹腔注射。每次激发时观察小鼠的表现。于末次激发24h处死小鼠，取右肺作苏木精．伊红(HE)染色病理切片，显微镜下观察炎症情况；收集左肺BALF，镜下计数白细胞总数、嗜酸性粒细胞(EOS)绝对数目，计算EOS百分比；用酶联免疫吸附法(ELISA)测定BALF中IL一25和IFN一的水平，并做相关性分析。结果哮喘组小鼠BALF中白细胞总数、EOS数目和百分比分别与正常对照组、地塞米松组比较均明显增加(P

3、高于正常对照组和地塞米松组(P<0．05)，而地塞米松组的含量也高于正常对照组；IFN一水平低于正常对照组和地塞米松组(P

4、andIFN．inBronchoalveolarLavageFluidofAsthmaticMiceLUwei，WANGLei，QIAOMing，WANGYu，JIANGJi—fu，WuZhong—ming(1．DepartmentofPediatrics，AfaliatedHospital，Zunyi563099，China；2．DepartmentofImmunology，ZunyiMedicalUnive~ity，Zunyi563003，China)ABSTRACTObjectiveToinvestigatethe

5、mechanismoftherapeuticactionofdexamethasoneonasthmaticmicebydetectingthelevelsofIL一25andIFN一inbronchoalveolarlavagefluid(BALF)．MethodsBalb／cmicewithSPFgradewererandomlydividedintonormalcontrolgroup，asthmagroupanddexamethasonegroup．Asthmagroupanddexamethasonegroup

6、weresensitizedandchallengedwithovalbumin(OVA)．Dexamethasonegroupwasintraperitoneallyinjectedwithdexamethasoneonehourbeforechallenging．Themicewereexecuted24hoursafterthelastchallenge，andtheHEstainedpathologicalsectionsoftherightlungweremade．Pathologicalsectionsofl

7、ungwereobserved．BALFintheleftlungwasalsocollected．Thetotalwhitebloodcellcountandabsoluteeosinophile(EOS)countwereobserved，andthepercentageofEOSwascalculated．ThelevelsofIL-25andIFN一weremeasuredwithELISA，andcorrelationanalysesweremade．ResultsThecountsoftotalwhitebl

8、oodcellandEOS，andthepercentageofEOSweresignificantlyhigherintheasthmagroupthaninthenormalcontrolgroupanddexamethasonegroup(P<0．05)．Nodifferenceswerefoundbetwee