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1、实用儿科临床杂志第21卷第21期2006年11月JApplClinPediatr,Vol.21No.21,Nov.2006·1459··论著·致敏和哮喘小鼠腹腔和支气管肺泡灌洗液巨噬细胞数量和功能表达121111张建华,王宇,徐敏,吴良霞,吴珉,王子才(1.上海交通大学附属第六人民医院儿科,上海200233;2.苏州市第二人民医院儿科,江苏苏州215006)ExpressionsandFunctionsofMacrophageofAbdominalCavityandBronchalveolarLavageFluidinSensitizedandAsthmaticM
2、iceZHANGJian-hua1,WANGYu2,XUMin1,WULiang-xia1,WUMin1,WANGZi-cai1(1.DepartmentofPediatrics,theSixthPeople′sHospitalAffiliatedtoShanghaiJiaotongUniversity,Shanghai200233,China;2.DepartmentofPediatrics,theSecondPeople′sHospitalofSuzhou,Suzhou215006,China)Abstract:ObjectiveToexploretheeff
3、ectsofantigenonexpressionsandfunctionsofmacrophages.MethodsMiceweresensitizedandchallengedwithovalgumin(OVA)byabdominalinjectionandinhalationtoestablishanallergicandasthmaticmodel.Macrophagesofthebron-cholveolarlavagefluid(BALF)andabdominalcavitywerecollectedandquantitychangesindiffer
4、enttimeswereobserved.Majorhistocompabilitycomplex(MHC)Ⅰaexpressionofabdominalcavitymacrophagesweredeterminedbyflowcytometry.Thelevelsoftumornecrosisfactor-α(TNF-α)ofabdominalcavitymacrophageculturesupernateweredetectedwithenzymelinkedimmunosorbentassay(ELISA).ResultsAfterchallenging,t
5、hesecond,fifth,seventhday,abdominalcavitymacrophages(×106/mL),totalBALFcells(×105/mL)andalveolarmacrophages(×105/mL)inallergicgroupwere7.59±1.27,2.01±0.61,1.66±0.52;19.43±6.37,2.87±0.50,1.84±0.40;7.68±2.28,1.40±0.45,0.99±0.38,re-spectively.Inasthmaticgroup,theywere16.62±5.56,3.63±0.79
6、,2.55±0.72;15.34±4.22,5.03±0.56,2.99±0.42;17.10±2.04,5.05±0.75,3.15±0.37,respectively.Theyweremorethanthoseofcontrolgroup(P<0.05).MHC-ⅠaexpressionsandTNF-α(μg/L)levelsofculturesupernatesofabdominalcavitymacrophagesinallergicandasthmaticgroupswere44.09%,59.28%;55.42±0.62,71.93±5.28.Asc
7、omparedwithcontrolgroup,theymarkedlyincreased(P<0.01).ConclusionsTheexpressionsofmacrophagesofabdominalcavityandBALFinallergicandasthmaticgroupmarkedlyincrease.TheexpressionsofMHC-ⅠaandTNF-αofmacrophagesmarkedlyincrease.JApplClinPediatr,2006,21(21):1459-1460Keywords:sensitivity;asthma
8、;macr
