黄芪多糖提取及对小叶杨幼苗叶绿素含量的影响-论文.pdf

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1、安徽农业科学，JournalofAnhuiAgn．Sei．2014，42(19)：6281—6284责任编辑高菲责任校对况玲玲黄芪多糖提取及对小叶杨幼苗叶绿素含量的影响邱本军，董婉莹，薛羿，葛康康，崔荣，张玉洲，马玲(东北林业大学林学院，黑龙江哈尔滨15oo40)摘要[目的]研究黄芪多糖最佳提取工艺，及其对小叶杨幼苗叶绿素含量的影响。[方法]运用水煎法提取黄芪多糖，采用固液比、提取溶剂、提取温度和提取时间4个试验条件，进行单因素试验，并利用正交试验设计对提取工艺进行优化，所得黄芪多糖处理小叶杨幼苗，探究黄芪多糖对其叶绿素含量的影响。[结果]单因素试验最终获得最佳提取工艺为以

2、5％醇碱为提取溶剂，固液比为1：15，提取温度为6o℃，提取1．5h。正交试验得出优化提取工艺为以5％醇碱为提取溶剂，固液比为1：10，提取温度为6o℃，提取1．0h。10mS／ml的黄芪多糖能够显著提高小叶杨叶绿素a和总叶绿素的含量，而0．1mg／ml黄芪多糖能够显著提高小叶杨叶绿素b的含量。[结论]黄芪多糖能够提高植物叶绿素含量，进而促进植物的生长发育。关键词黄芪多糖；正交试验；小叶杨；叶绿素含量中图分类号$567文献标识码A文章编号0517—6611(2014)19—06281—04ExtractionofAstragaluspolysaccharideandItsI

3、mpactonTheChlorophyllContentofPopulussimoniiQIUBen-jun，MALiIIgetal(SchoolofForestry，NortheastForestryUniversity，Harbin，Heilongjiang150040)Abstract『0biective]ThebestextractionprocessofAstragalusp0lysaccharideanditsimpactonchlorophyllcontentofpopalussimoniiseed．1ingswerestudied．[Method]Using

4、boilingmethodtoextractAstragaluspolysaecharidebysinglefactorexperimentsun．dersolid—liquidratio，solventextraction．extractiontemperatureandextractiontimeinfourexperimentalconditionssinglefactorexperiments．Byorthogonalexperi-mentaldesigntooptimizetheextractionprocess．Astragaluspolysaccharidep

5、rocessingPopulussimoniisaplings，whichinvestigatetheeffectsofastragaluspolysaccharidesonchlorophyllcontentofPopulussimonii．『ResultJUltimatelygetthebestextractionprocessasfollows：5％alcoho1solutionofanalkaliasextractionsolvent，solid—liquidratioof1：15，extractiontemperatureis60℃，extraction1．5h．

6、Tooptimizetheex—tractionprocesswasobtainedwitll5％alcoholsolutionofanalkaliasextractionsolvent。solid—liquidratioof1：10，extractiontemperatureiS6O℃．extraction1h．TheresultsshowthatAPS10mg／mlcansignificantlyincreasethecontentofchlorophyllaandtotalchlorophyll，and0．1mg／mlAstragaluspolysaceharides

7、cansignificantlyimprovesimonies’chlorophyllbcontent．[Conclusion]AstragaluspolysaccharidescanimproveplantchlorophyllCOnfent，thuscontributingtOthegrowthanddevelopmentofplants．KeywordsAstragaluspolysaccharide；Orthogonalexperiments；Populussimonii；Chlorophyllconten