细胞毒性T淋巴细胞相关抗原-4基因多态性与Graves病复发的相关性研究.pdf

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1、14JBengbuMedCoil，January2015，Vo1．40，No．1[文章编号]1000-2200(2015)01-0014-03·基础医学·细胞毒性T淋巴细胞相关抗原4基因多态性与Graves病复发的相关性研究查秀婧，毕娅欣[摘要]目的：探讨细胞毒性T淋巴细胞相关抗原(CTLA)-4基因多态性与Graves病(GD)复发的相关性。方法：选择102例GD复发患者(A组60例为停药3年内复发60例，B组为3年后复发42例)、4O例GD初发患者和8O名健康人群(正常对照组)，应用聚合酶链反应一限制性内切酶片段长度多态技

2、术进行CTLA-4基因外显子1第17密码子49位点A／G多态性检测。结果：CTLA一4基因第一外显子49位点基因型及等位基因的频率分布显示，GD初发组患者GG基因型频率及G等位基因频率均高于正常对照组(P<0．01)。GD复发A组GG基因型频率均高于B组和初发组(P<0．01)，B组GG基因型频率高于GD初发组(P<0．01)。结论：CTLA-4基因外显子1第17密码子49位点A／G多态性与GD的发生存在相关性，该位点GG基因型可能是GD患者对抗甲状腺药物疗效差及早期复发的原因。[关键词】Graves病；细胞毒性T淋巴细胞相关

3、抗原4；复发；基因多态性[中图法分类号]R581．1[文献标志码]ADoI：10．13898／j．cnki．issn．1000-2200．2015．01．005ThecorrelationofthepolymorphismofcytotoxicTlymphocyteassociatedantigen-4geneandrecurrenceofGravesdiseaseZHAXiu-jing，BIYa—xin(j．DepartmentofEndocrinology，TheSecondAffiliatedHospitalofBeng

4、buMedicalCollege，BengbuAnhui233040；2．DepartmentofEndocrinology，TheFirstAfiliatedHospitalofBengbuMedicalCollege，BengbuAnhui233004，China)[Abstract]Objective：ToexplorethecorrelationofthepolymorphismofcytotoxicTlymphocyteassociatedantigen-4(cTLA_4)geneandrecurenceofGrav

5、es’disease(GD)．Methods：TheA／GpolymorphismsofCTLA_4geneexonseventeenthcodon49locusin102patientswithGDrecurence,whoincluded60recurrencecaseswithin3yearsofwithdrawaldrug(Agroup)and42recurencecasesafter3yearsofwithdrawaldrug(Bgroup)，40primaryGDpatients(primarygroup)and8

6、0healthypeople(normalcontrolgroup)weredetectedusingpolymerasechainreactionrestrictionfragmentlen~hpolymorphism．Results：ThefrequencydistributionofgenotypeandalleleinCTLA-4geneexon1of49locusshowedthatthefrequencyofGGgenotypeandGalleleinprimaryGDpatientswerehigherthant

7、hoseinnormalcontrolgroup(P<0．O1)，thefrequencyofGGgenotypeinAgroupwashigherthanthatinBgroupandprima~group(P<0．O1)，andthefrequencyofGGgenotypeinBgroupwashigherthanthatinprima~group(P<0．01)．Conclusions：TheA／GpolymorphismofCTLA-4geneexonseventeenthcodon49locusisrelatedt

8、otheGDoccurrence，theGGgenotypeofwhichmaybethereasonofthepooreffectofantithyroiddrugsandearlyrecurenceinGDpatients．[Keywords]Gravesdisease；