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1、CTLA-4基因微卫星多态性与广西地区汉族Graves病的相关性研究第17卷第21期2007年l1月中国现代医学杂志ChinaJournalofModernMedicineV01.17No.21NOV.2oo7文章编号:1005—8982(2007)21—2609—04CTLA一4基因微卫星多态性与广西地区汉族Graves病的相关性研究木罗佐杰,谢新荣,秦映芬,马燕.,邝晓聪,梁杏欢(1.广西医科大学第一附属医院内分泌科广西南宁530021;2.广西民族医院广西南宁530000;3.广西医科大学第一附属医院超声诊断科广西南宁5300214.广
2、西医科大学病理生理教研室,广西南宁530021)?论着?摘要:目的研究CTLA一4基因微卫星多态性与广西地区汉族GrRyes病(Gr¨eStDisease.GD)的相关性.方法64例广西地区汉族GD患者和50例正常对照组,应用聚合酶链反应技术,检测CTLA一4基因外显子4的3,非翻译区包含(AT)n[CTLA一4(AT)n1重复序列的特异性等位基因.结果广西地区汉族人CTLA一4(AT)n有18种等位基因;汉族GD组与正常对照组比较,106bp等位基因在GD组中显着增高(P<0.05),汉族正常对照组104bp等位基因频率较GD组高(P
3、=0.01),差异有统计学意义.结论CTLA一4基因与广西汉族GD患者明显相关,CTLA一4(AT)n106bp等位基因可能是广西汉族GD的易感基因,104bp等位基因可能是广西汉族GD的保护基因.关键词:Gravest病;CTLA一4基因;微卫星多态性;易感基因/保护基因中图分类号:R593.2文献标识码:AStudiesontheassociationofCTLA-4genemicrosatellitepolymorphismwithGravesdiseaseofHannationalityinGuangxiprovinceLUOZuo-
4、jie,XIEXin—rongz,QINYing-fen,MAYan.,KUANGXiao-cong,HANGXing-huanf1.TheDepartmentofEndocrinology,theFirstAffiliatedHospitdofGuangxiMedicalUniversity,Nanning,Guangxi530021,P_尺.China,"2.GuangxiNationHospital,Nanning,Guangxi530000,P.R.China,"3.TheDepartmentofUltrasoundDiagnosis
5、,theFirstAffiliatedHospitdofGuangxiMedicalUniversity,Nanning,Guangxi530021,P.R.China,".TheDepartmentofPathophysiology,GuangxiMedicalUniversity,Nanning,Guangxi530021.P.尺.China)Abstract:【Objecfive】Toinvestigatetherelationshipbetweenthepolymorphic(AT)nrepeatsinthe3untrans—late
6、dregionofexon4ofCTLA一4gene[CTLA-4(An]andGravesdisease(GD)ofHannationalityinGuangxiprovince.【Methods】Thestudygroupcomprised64GDpatientsand50normalcontrols,matchedwithpatientsforsex,andage.AmplificationoftargetDNAwascarriedoutbythepolymerasechainreaction(PCR],withprimers5,_GC
7、CAGTGATGCTAAAGGTTG一3and5'-AACATACGTGGCTCTATGCA-3.Theamplifiedproductswereelectrophoresedby8%nondenaturingpolyacrylamidgeland~Howedby0.1%silverstaining.Someoftheamplifiedproductsweresequenceddirectly.【Results】18allelesofCTLA一4(AnwerefoundinGuangxiHannationalityindividuals.10
8、6bpapparentlyincreasedinpatientswithGDinHannationalitycomparedwithhealthycontrols.
