肾上腺切除幼鼠癫痫易感性及海马CRHmRNA的表达.pdf

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1、实用医学杂志2015年第31卷第7期肾上腺切除幼鼠癫痫易感性及海马CRHmRNA的表达王雅洁邹丽萍摘要目的：探讨肾上腺切除幼鼠癫痫易感性及对海马促肾上腺皮质激素释放激素(CRH)mRNA表达的影响。方法：60只WistarPIO幼鼠随机均分为空白对照组、肾上腺切除组、假手术组。手术次日腹腔注射NMDA7mg／kg诱发抽搐，评价发作潜伏期(min)及程度。原位杂交方法检测海马CRHmRNA表达。结果：空白对照组潜伏期、评分为(43．65±2．96)min、4．40±0．60，肾上腺切除组为(35．05±2．35)min、5．56±0．76，假手术组为(42．604-1

2、．90)min、4．55±0．76。肾上腺切除组与其他2组比较，潜伏期、评分差异具有显著性(F=73．73，P<0．05；F=15．52，P<0．05)，3组幼鼠海马CRHmRNA阳性表达率为20％、55％、15％，与其他2组相比，肾上腺切除组海马CRHmRNA增加具有显著意义(x=9．048，P<0．05)。结论：肾上腺切除幼鼠潜伏期缩短、评分增加，海马CRHmRNA提高，提示肾上腺切除幼鼠对致痫剂NMDA敏感性增高。关键词痉挛，婴儿；肾上腺切除；肾上腺糖皮质激素释放激素；N一甲基一D一天冬氨酸Efectofbilateraladrenalectomyonthes

3、usceptibilitytoNMDA·inducedseizureandhippocampalCRHmRNAinyoungratsWANGYa-jie，ZOULi-ping．DepartmentofPediatrics，Bering~autanHospital，CapitalMedicalUniversity，Beijing100050，ChinaCorrespondingauthor：ZOULi-pingEmail：zouliping21@hotmail．com【Abstract】ObjectiveTodemonstratetheeffectofbilatera

4、ladrenalectomy(ADX)onthesusceptibilitytoNMDA—inducedseizureandhippocampalCRHmRNAinyoungrats．Methods60WistarratsatPIOweredividedintocontrolgroup，ADXgroupandShamADXgroup．Inthenextdayafteroperation，7mg／kgNMDAwasi~ectedtoinduceseizure，andincubationperiod(inminutes)aswellasdegreewasevaluate

5、d．InsituhybridizationwasusedtodetecthippocampalCRHmRNAexpression．ResultsLatencywas(43．65±2．96minutesincontrolgroup，(35．05±2．35)minutesinADXgroupand(42．60±1．90)minutesinSham—ADXgroup．LatencyintheADXratsincreasedsignificantly(F=73．73，P<0．05)．Theseizurescalewas(4．40±0．60)incontrolgroup，(5

6、．56±0．76)inADXgroupand(4．55±0．76)inSham—ADXgroup．TheseverityofseizuresinADXgroupincreasedsignificantlycomparedwiththecontrolandSham—ADXgroups(F=15．52，P<0．05)．CRHmRNAexpressionwas20％incontrolgroup，55％inADXgroupand15％inSham-ADXgroup．CRHmRNAintheADXgroupwassignificantlyelevated(x=9．048，P<

7、0．05)．ConclusionAdrenalectomyexacerbatesNMDA—inducedspasmseizuresinyoungrats，whichmightberelatedtoCRHmRNAexpressioninthehippocampus．【Keywords】Spasms，infantile；Adrenalectomy；Corticotrophin—releasinghormone；N—Methyl—D—aspartate婴儿痉挛症由West于1841年首次描述，是一前认为促肾上腺皮质激素释放激素(corticotrophin—种较为严重