母爱剥夺对大鼠行为学及癫痫易感性的影响

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1、母爱剥夺对大鼠行为学及癫痫易感性的影响摘要目的研究母爱剥夺对大鼠行为学及随后癫痫发作的影响。方法将新生的SpragueDawle(SD)雄性大鼠随机分为母爱剥夺组和对照组。母爱剥夺组大鼠自出生后第2天至第14天，每天上午9：00．12：00给予母爱剥夺，对照组不给予任何处理；第21天断奶分笼，监测体重及每日体重增长量；第26天开始进行糖水偏爱实验：糖水偏爱实验结束后，以上两组大鼠随机分为两组，其中一组接受腹腔注射匹鲁卡品建立癫痫模型(C+P组和M+P组)，另一组给予同等剂量的生理盐水腹腔注射(C+C组和M+C组)；采用Ne

2、uN免疫荧光染色法计数大鼠海马齿状回颗粒层细胞数。结果母爱剥夺组大鼠体重及每日体重增长量显著低于对照组(P

3、W海马齿状回颗粒层细胞数较对照组(C+C组)显著增加(PO．05)。结’论母爱剥夺应激可以造成大鼠成年后出现类似抑郁样行为学改变，并使癫痫发作易感性增加。硕士研究生：潘楠楠(神经病学)指导教师：郑乃智关键词：母爱剥夺；糖水偏爱；抑郁；癫痫；颗粒层TheeffectsonbehavioralandsusceptivitytoepilepsyofratsbymaternaldeprivationAbstractObject

4、iveWrestudiedtheeffectsofmatemaldeprivation(MD)onbehavioralandsusceptivityto．epilepsyfollowedMDofrats．MethodsNewbornSDmaleratsweredividedintomaternaldeprivationgroupandcontrolgroup(C)randomly；RatsinMDgroupexperiencedmaternaldeprivationfor3hourseachdayfromthe2ndtot

5、he14thpostnatalday(PD)；RatsinCgroupdidn’treceiveanytreatment；AtPD21．allratswereweaned，andweigheddailyandrecordedtheincreaseofbodyweighteveryday；AllratsreceivedsucroseconsumptiontestatPD26；Eachgroupdividedintotwogroupsrandomly，onegroupofratsreceivedpilocarpinetoind

6、uceseizures(M+PandC+P)，whiletheothergroupreceivednormalsalineinsteadofpilocarpine(M+CandC+C)；Itwas‘countedthenumberofneuronsathippocampaldentategymsgranularlayerofratsbyNeuNimmunofluorescencestain．ResultsbodyweightsanditsincreasesofratsinMDgroupweresignificantlowe

7、rthanratsinCgroup(P<0．05)；ItwassignificantlylowersucrosepreferencerateofratsinMDgroupthaninCgroup(P<0．05)；Afterreceivedpilocarpine．thelatencysofseizuresinMDratswassignificantlyshortencomparedtoratsinCgroup：TheseverityofseizuresofratsinMDgroupwashigherthanratsinCgr

8、oupbyRacine’sscale；Thenumberofneuronsinhippocampaldentategyrusgranularlayerlwand4waftermaternaldeprivationofratsinM+Cgroupweresignificantlydecreasedcomp