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1、人参皂甙-Rh2对人乳腺癌MCF7/AdrR细胞增殖和凋亡的影响EfficacyofGensenosideRh2onproliferationandapoptosisforMCF7/AdrRhumanbreastcellline摘要:目的?研究人参皂甙-Rh2(GS-Rh2)对乳腺癌MCF7/AdrR细胞增殖和凋亡的影响。方法?以MCF7/AdrR细胞不加药物干预,作为对照组;以Rh2单独作用于MCF7/AdrR细胞,作为Rh2组;以低浓度阿霉素(ADM)单独作用于MCF7/AdrR细胞,作为ADM组;以Rh2和阿霉素联用于MCF7/AdrR细胞,
2、作为Rh2+ADM组。用MTT比色法观察GS-Rh2对体外培养的MCF7/AdrR细胞生长的抑制作用;用荧光显微镜观察用药前后MCF7/AdrR细胞的形态学变化;用流式细胞仪检测凋亡细胞和细胞周期变化;用Westernblot的方法检测Fas、Bax、Bcl-2及Bcl-xL蛋白表达的变化。结果?MCF7/AdrR细胞经GS-Rh2作用后,生长受抑制,呈剂量和时间依赖性;显微镜显示,经GS-Rh2作用后,MCF7/AdrR细胞呈较明显的凋亡形态学改变,GS-Rh2能将细胞周期阻滞于G0/G1期,同时可能参与促凋亡蛋白Fas、Bax表达升高及抑制凋亡
3、蛋白Bcl-2表达降低;但在GS-Rh2处理前后Bcl-xL无明显变化。结论?GS-Rh2能抑制体外培养的MCF7/AdrR细胞增殖并诱导其凋亡,且这种作用可能是通过上调Fas、Bax表达、下调Bcl-2表达以及阻滞细胞周期而实现。关键词:人参皂甙-Rh2;乳腺癌;细胞凋亡中图分类号:R737?9;R979?1???文献标识码:A文章编号:1001-6821(2010)12-0923-05Abstract:Objective?ToexaminetheeffectofGensenosideRh2(GSRh2)onproliferationandapo
4、ptosisMCF7/AdrRhumanbreastcellline?Methods?MCF7/AdrRcellsweredividedintocontrolgroup,addedwithnormalsaline;ADMgroup,addedwithadriamycin(ADM)atthelowcon?centration;Rh2group,addedwithRh2;andADM+Rh2group,addedwithADMatthelowconcentrationandRh2?CellgrowthinhibitionwasevaluatedbyMT
5、Tcolorimetricassay?Cellularmorphologywasobservedundermicroscope?Apoptoticcellsandthechangesofcellcyclewerede?terminedbyflowcytometry?WesternblotwasusedtodetecttheproteinexpressionofFas,Bax,Bcl-2andBcl-xL?Results?TheinhibitionofGS-Rh2onthegrowthofMCF7/AdrRcellswasinatime-anddos
6、e-dependentmannerbyMTT?GS-Rh2wascapableofarrestingthecellcycleatG0/G1phase?Pro-apoptoticfactors,FasandBaxwerere?markablyhigherthancontrolcells,whiletheanti-apoptoticfactors,Bcl-2weresignificantlylowerthancontrolcells,buttheexpressionofBcl-xLproteindidnotchangesignificantly?Con
7、clusion?GS-Rh2exhib?itedgrowthinhibitioneffectandinducedapoptosisonMCF7/AdrRcelllineinvitro?ApoptosisofMCF7/AdrRcellsinducedbyGS-Rh2mayberel?atedwitharrestingcellcycle,up-regulatingFas,Baxproteinexpressionanddown-regulatingmutatedBcl-2pro?teinexpression?Keywords:GensenosideRh2
8、;breastcancer;apoptosiscell??近年研究发现[1,2],人参具有抗癌活性,其主要活性成分人参皂苷
