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时间:2020-05-14
《抗人脊索瘤杂交瘤细胞系的建立及其单抗在病理诊断中的应用.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、现代肿瘤医学2010年12月·2297·ofdelayed0nsetdiarrheainrats『J].CancerChemotherPharma—[17]ShinoharaH,BucanaCD,KillionJJ,eta1.Intensifiedregres—col,2000,46(3):211—220.sionofcoIoncancerlivermetastasesinmicetreatedwithirinote—[13]ChiuCT.Intestinalmueosallesioninlowflowstates[J].
2、ArchcanandtheimmunomodulatorJBT3002fJi.JImmunother,Surg,1970,101:478—4852000,23(3):321—331.【l4]KehrerDF,SparreboomA,VerweijJ.Modulationofirinoteean—[18]SHRosenof.OctreotideLARresolvesseverechemotherapy—io—induceddiarrheabycotreatmentwithneomycinincancerpatientsduc
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4、erapywithconcomitantradiosensitizing3441.5一FUchemotherapyfortreatmentofneuroendocrinetnmors:Pre。[16]GovindamianR.Irinotecanandthalidomideinmetastaticcolorec.liminaryexperience[J].Cancer,2009,24(5):527—533.talcancer[J].Oncology(Huntingt),2000,14(12Suppl13):(编校:张志明)
5、29】抗人脊索瘤杂交瘤细胞系的建立及其单抗在病理诊断中的应用刘云燕,杨连甲,裘秀春,文艳华,张殿忠,范清宇Establishmentofanti——chordomahybridomacelllineanditsapplicationinthepathologi_caldiagnosisofhUmanchordomaLIUYun—yan,YANGLian—jia,QIUXiu—chun,WENYan—hua,ZHANGDian—zhong,FANQing—yuOrthopaedicOncolgyInstituteofPLA,
6、TangduHospital,theFourthMilitaryMedicalUniversity,Xi"an710038,China.【Abstract】0bjective:Toestablishahybridomacelllinesecretinganti—chordomamonoelonalantibody,andstudyitsbindingreactivityinhumanchordomaandotherbonetumortissues.Methods:Usinghybridomatechnology,topre
7、—DareMAb一6F2hybridomacelllinethroughimmuningBALB/CmousewithhumanchordomacelllineCM一319.ThenthebindingreactivityofMAb一6F2wasdetectedwithimmunohistochenlistryinfonrdifierentbonetunlorparaf-finsectionsandnormalnucleuspulposustissues.Results:TheBindingrateofMAb一6F2was
8、10%inosteosarcomatissue,10%inchondrosarcomatissue,10%inosteoidosteomatissue,andnegativeinnormalnucleuspulposustis—sue,but100%inchordomatissue.Thebinding
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