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时间:2020-05-09
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1、东南大学学报医学版JSoutheastUnivMedSciEdi、2一o15,Jun;34(3)/:‘3一93一—3一96uSoutheastUniv(MedE·393·factor,SCF)的作用。SCF是c—kit的天然配体,对ICe[6]JAIND,MOUSSAK,TANDONM,eta1.Roleofinterstitial正常生长、表型维持、细胞网络的稳定起着重要作cellsofcajalinmotilitydisordersofthebowel[J].AmJGas-用¨引。本实验中,在培养基中加入了5ng·ml的troenterol,2003,98(3):618
2、—624.[7]TONGWD,LIUBH,ZHANGLY,eta1.Expressionofc。kitSCF对原代ICC成功培养至关重要。messengerribonucleicacidande‘kitproteininsigmoidcolon本实验成功地对胆囊原代ICC进行分离、培养和ofpatientswithslowtransitconstipation[J].IntJColorectal鉴定,为今后进一步研究ICe的生理特性以及其在胆Dis,2005,20(4):363。367.囊结石发病中的作用机制研究提供了基础。然而,该[8]CHENZH,ZHANGYC,JIA
3、NGWF,eta1.Characterization种方法培养的ICC没有达到较高的纯度,目前有学者ofinterstitialCaja1.progenitorscellsandtheirchangesin通过流式细胞仪和免疫磁珠法来纯化肠道ICC。我们Hirsehsprung’sdisease[J].PLoSOne,2014,9(1):e86100.将继续研究,以摸索出一套高效、高纯的胆囊原代ICC[9]KIYOHARAT,SHINOMURAY,ISOZAKIK,eta1.Ade—培养方法。creasednumberofc。kit‘。expressingcellsinap
4、atientwithaf-ferentloopsyndrome[J].JGastroenterol,2003,38(4):[参考文献]390-394.[1]HUIZIMGAJD,CHENJ.InterstitialcellsofCajal:updateon[10]朱丽娅,嵇振岭,赵刚.完全腹腔镜联合胆道镜保胆手术basicandclinicalscience[J].CurrGastroenterolRep,2014,的护理配合[J].现代医学,2014,42(2):194—196.16(1):363.[11]PASTERNAKA,GILK,MATYJAA,eta1.Loss
5、ofgallblad—[2]LAVOIEB,BALEMBAOB,NELSONMT,eta1.Morphologi—derinterstitialCaja。likecellsinpatientswithcholelithiasiscalandphysiologicalevidenceforinterstitialcellofCajal—like[J].NeurogastroenterolMotil,2013,25(1):17—24.cellsintheguineapiggallbladder[J].JPhysiol,2007,579[12]PASTERNAKA,GAJDAM,
6、GILK,eta1.Evidenceofinter-(2):487—501.stitialCajal—likecellsinhumangallbladder[J].FoliaHisto—『31SANDERSKM.InterstitialcellsofCajalattheclinicalandchemCytobiol,2012,50(4):581。585.scientificinterface[J].JPhysiol,2006,576(3):683·687.[13]XUWD,JIANGX,LANL,eta1.Long—termcultureand[4]WARDSM,SANDE
7、RSKM.Physiologyandpathophysiologycryopreservationofinterstitialcellsofcaja1[J].ScandJGas—oftheinterstitialcellofCajal:frombenchtobedside.I.Func—troenterol,2012,47(1):89—98.tionaldevelopmentandplasticityofinterstitialcellsofCajal[14]TONGW,JIAH,ZHANGL,eta1.Exoge
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