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时间:2020-05-07
《不同类型HBV感染人群T细胞对HBV抗原蛋白免疫应答的差异分析-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、·984·免疫学杂志2013年11月第29卷第11期IMMUNOLOGICALJOURNALVo1.29No.11Nov.2013·论著·【文章编号】1ooo一8861(2013)11-0984—04不同类型HBV感染人群T细胞对HBV抗原蛋白免疫应答的差异分析叶红珍,李志勇【摘要】目的探讨不同类型乙肝病毒(HBV)感染者免疫T细胞群(CD4T和CD8T)对乙肝病毒抗原蛋白免疫应答的特点及差异性。方法选取107例HBV感染患者为研究对象,根据患者病情分为HBV携带组(A组)32例、急性乙肝组(B组)38例、慢性乙肝组(C组)37例,同时选取既往HBV组41
2、例作为对照组,分别对上述4组患者应用免疫荧光分析仪检测免疫T细胞对HBeAg、HBcAg、HBsAg等HBV特异性抗原蛋白的免疫应答。结果急性乙肝患者ALT、AST、总胆红素含量以及慢性乙肝组患者HBVDNA含量显著高于其他各组,差异具有统计学意义(P<0.05)。反应频率分析:急性乙肝患者对HBV常见的3种抗原蛋白阳性反应频率与慢性乙肝患者相比无统计学意义。HBV携带组对HBeAg的反应频率显著高于HBcAg及HBsAg3、显著高于其余各组(P<0.05),A、C相比无统计学意义,对照组高于A组(尸B组>C组>A组,两两相比差异有统计学意义(P<0.05)。3)HBsAg反应强度:B组>C组>A组=对照组。4)A组组内对比:HBeAg反应强度高于HBsAg及HBcAg(P<0.05).HBsAg与HBcAg相比无统计学意义(P>O.05)。5)B组组内对比:HBeAg>HBsAg>HBcA比较有统计学意义(P4、意义(P>0.05)。结论通过检测HBV感染者T细胞群(CD4T和CD8T)对乙肝病毒抗原蛋白免疫应答可预测患者病情的发展,可为HBV诊断提供指导性依据。【关键词】乙肝;HBV感染;T细胞;抗原蛋白[中图分类号】R392—33[文献标识码】AThevarianceanalysisofimmuneresponsesbetweendiferentHBVantigensanddiferenttypesofTcellsinHBV-infectedpeopleYEHongzhen,LIZhiyongClinicalLaboratory,FirstAffiliated5、HospitalofXiamenUniversity,Xiaman361022,ChinaCorrespondingAuthor:YEHongzhen,E-mail:352162644@qq。com[Abstract]ToexplorethecharacteristicsanddifferencesofdifferenttypesofhepatitisBvirusfHBV)infectionbyobservingimmunereponsebetweenTcellandHBVantigenprotein,107casesofHBVinfectionpatie6、ntsweredividedintoHBVcarriergroupA(n=32),acutehepatitisgroupB=38),chronichepatitisgroupC(n=37),withpreviousHBVpatients(n=41)ascontrols.TcellsandtheimmuneresponseofHBeAg,HBcAg,HBsAgof4groupswereanalyzedbyimmunefluorescenceanalyzer.GeneralsituationanalysisshowedthatALTAST,andtotal,b7、ilirubinlevelsinpatientswithacutehepatitisBandHBVDNAcontentinpatientswithchronichepatitisBweresignificantlyhigherthanthoseinothergroupsfP<0.05).WhileresponsefrequencyanalysisillustratedthatpositivereactionfrequencyofthreecommonHBVantigenshasnostatisticaldifferenceinpatientswithacu8、tehepatitisBandchronichepatitisB:
3、显著高于其余各组(P<0.05),A、C相比无统计学意义,对照组高于A组(尸B组>C组>A组,两两相比差异有统计学意义(P<0.05)。3)HBsAg反应强度:B组>C组>A组=对照组。4)A组组内对比:HBeAg反应强度高于HBsAg及HBcAg(P<0.05).HBsAg与HBcAg相比无统计学意义(P>O.05)。5)B组组内对比:HBeAg>HBsAg>HBcA比较有统计学意义(P4、意义(P>0.05)。结论通过检测HBV感染者T细胞群(CD4T和CD8T)对乙肝病毒抗原蛋白免疫应答可预测患者病情的发展,可为HBV诊断提供指导性依据。【关键词】乙肝;HBV感染;T细胞;抗原蛋白[中图分类号】R392—33[文献标识码】AThevarianceanalysisofimmuneresponsesbetweendiferentHBVantigensanddiferenttypesofTcellsinHBV-infectedpeopleYEHongzhen,LIZhiyongClinicalLaboratory,FirstAffiliated5、HospitalofXiamenUniversity,Xiaman361022,ChinaCorrespondingAuthor:YEHongzhen,E-mail:352162644@qq。com[Abstract]ToexplorethecharacteristicsanddifferencesofdifferenttypesofhepatitisBvirusfHBV)infectionbyobservingimmunereponsebetweenTcellandHBVantigenprotein,107casesofHBVinfectionpatie6、ntsweredividedintoHBVcarriergroupA(n=32),acutehepatitisgroupB=38),chronichepatitisgroupC(n=37),withpreviousHBVpatients(n=41)ascontrols.TcellsandtheimmuneresponseofHBeAg,HBcAg,HBsAgof4groupswereanalyzedbyimmunefluorescenceanalyzer.GeneralsituationanalysisshowedthatALTAST,andtotal,b7、ilirubinlevelsinpatientswithacutehepatitisBandHBVDNAcontentinpatientswithchronichepatitisBweresignificantlyhigherthanthoseinothergroupsfP<0.05).WhileresponsefrequencyanalysisillustratedthatpositivereactionfrequencyofthreecommonHBVantigenshasnostatisticaldifferenceinpatientswithacu8、tehepatitisBandchronichepatitisB:
4、意义(P>0.05)。结论通过检测HBV感染者T细胞群(CD4T和CD8T)对乙肝病毒抗原蛋白免疫应答可预测患者病情的发展,可为HBV诊断提供指导性依据。【关键词】乙肝;HBV感染;T细胞;抗原蛋白[中图分类号】R392—33[文献标识码】AThevarianceanalysisofimmuneresponsesbetweendiferentHBVantigensanddiferenttypesofTcellsinHBV-infectedpeopleYEHongzhen,LIZhiyongClinicalLaboratory,FirstAffiliated
5、HospitalofXiamenUniversity,Xiaman361022,ChinaCorrespondingAuthor:YEHongzhen,E-mail:352162644@qq。com[Abstract]ToexplorethecharacteristicsanddifferencesofdifferenttypesofhepatitisBvirusfHBV)infectionbyobservingimmunereponsebetweenTcellandHBVantigenprotein,107casesofHBVinfectionpatie
6、ntsweredividedintoHBVcarriergroupA(n=32),acutehepatitisgroupB=38),chronichepatitisgroupC(n=37),withpreviousHBVpatients(n=41)ascontrols.TcellsandtheimmuneresponseofHBeAg,HBcAg,HBsAgof4groupswereanalyzedbyimmunefluorescenceanalyzer.GeneralsituationanalysisshowedthatALTAST,andtotal,b
7、ilirubinlevelsinpatientswithacutehepatitisBandHBVDNAcontentinpatientswithchronichepatitisBweresignificantlyhigherthanthoseinothergroupsfP<0.05).WhileresponsefrequencyanalysisillustratedthatpositivereactionfrequencyofthreecommonHBVantigenshasnostatisticaldifferenceinpatientswithacu
8、tehepatitisBandchronichepatitisB:
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