Prohibitin 3’-UTR基因多态性与粤东乳腺癌发病风险的研究.pdf

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1、癌变■变窑变乙第25卷第4期圄■圈ProhibitinY．untranslatedregion尸hibitin3’一UTRpolymorphismandbreastcancer基因多态性与粤东00■·10乳腺癌发病风险risklneasternOuangdong1-1-的研究oOfsouthernCGhina彭琳／黄裔腾’／罗育豪f1．汕头大学医学院附属肿瘤医院检验科，广东PENGLin，HUANGYi—te一，LUOYu-hao汕头515041；2．汕头大学医学院肿瘤研究(1．ClinicalLabora

2、to~CancerHospitalofShantouUnivers中心，广东汕头515041)MedicalCollege,Shantou515041,"2CancerResearchCenter,ShantouUniversityMedicalCollege,Shantou515041，GuangdongC㈧【摘要】目的：探$~Prohibitin(PHB)3'-非翻译N~"(untranslatedregion，UTR)基因多态性与粤东汉族女性乳腺癌易感性的关系。方法：采用病例一对照研究，收集231例乳

3、腺癌患者和169例健康对照，提取血液基因组DNA，利用高分辨率熔解曲线(highresolutionmelting，HRM)法7(~PHB~因单核苷酸多态性位~1049620、rs9893420、~4987083进行基因分型，并对检测结果进行非条件Logistic归分析和X检验。结果：PHB基因rs9893420和rs4987083位点在病例组和对照组人群均表现为单一基因型，rs1049620三种基因型G／G、A／G、A，A在病例组和对照组间分布差异均无统计学意义(尸>0．05)，在不同组织病理学分型和不同

4、激素受体表达病例中分布差异亦无统计学意义(P>0．05)。结论：未发现P瑚基因3’一UTRSNP与粤东汉族女陛乳腺癌易感性存在关联。【关键词】乳腺癌；Prohibitin；单核苷酸多态性；高分辨率熔解曲线中图分类号：R730．231文献标识码：A文章编号：1004—616X(2013)04—0293—04doi：10．3969／j．issn．1004—616x．2013．04．011[ABSTRACT]OBJECTIVE：TodeterminetheassociationofProhibitin(PHB)3

5、’一untranslatedregion(UTR)polymorphismwiththeriskofbreastcancerineasternGuangdongofsouthernChina．METHODS：Acase—controlstudyof231breastcancerpatientsand169healthycontrolswereenrolledintothisstudy．GenomicDNAwasextractedfromwholebloodcells．Singlenucleotidepoly

6、morphismsofrs1049620，rs9893420andrs4987083weredeterminedbythehighresolutionmelting(HRM)method．DatawereanalyzedviaLogisticregressionanalysisandChi—squaretest．RESULTS：PHBgeneatrs9893420andrs4987083sitesshowedsinglegenotypeinbreastcancergroupandcontro1．Therew

7、erenosignificantdifferencesinfrequenciesofG／GA／Gand

8、atrs1049620betweenpatientsandcontrols(P>0．05)．Meanwhile，nosignificantcorrelationcouldbefoundbetweenrs1049620SNPandclinico—pathologiccharacteristicsofbreastcancersuchashistologicalclassificationandexpressi

9、onofhormonereceptor．CONCLUSION：Ourresultsdonotlendsuppo~tothehypothesisthatPHBpolymorphismcontributestosusceptibilityofbreastcancer．【KEYWORDS]breastcancer；Prohibitinsinglenucleotidepolymophism；highresolutionm