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页数:7页
时间:2020-04-30
《利用响应面优化Klebsiella variicola对麻疯树籽饼粕佛波酯的固态发酵降解.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、!:!:!!:!!:!:!:●墨盈盈盈盈盈盈_囫墨盔应用与环境生物学报ChinJApplEnvironBiol2014,20(3):431—438佛波酯的固态发酵降解术刘莹张晓喻白艳林文瑜尹晶唐琳料叫』I1人学i命科学学院成都6101)64能源柑物物燃料f}fJ制备及利用闻家地方联合12程实验室成都610225国日为降解麻疯树籽饼粕中的毒性成分佛波酯,利用Klebsiellavariicola进行固态发酵.采用单因素实验法研究了发酵温度、接种量、初始加:,K量、发酵时间对佛波酯降解率的影响.在此实验结果基础上,利用DesignExpert8.0软件进行分析建立了发酵温度、接种量、初始加水
2、量3个因素对佛波酯降解率影响的优化模型.该模型极显著(P<0.001),拟合度良好.经实验证实,在发酵温度为37℃、接种量33%(V/m)、初始加水量为105%(Vim)条件下发酵48h,佛波酯降解率可达到84.30%,另一种毒性物质curcin的降解率可达63.21%.研究表明,利用响应面优化K.variicola固态发酵麻疯树籽饼粕过程,既可提高佛波酯降解率,也可降解curcin,具有生产指导意义.图3表9参29笺淘麻疯树籽饼粕;佛波酯;Klebsiellavariicola;固态发酵;curcinCLCQ949.753.506:X172Optimizationofresponses
3、urfacefordegradingphorbolestersinJatrophacurcasseedcakethroughKlebsiellavariicolasolid.statefermentation水LIUYing,ZHANGXiaoyu,BAIYanlin,WENYu,YINJing&TANGLin一C'olleget日{啦Sciences.SichuanUr#versiO,、Chengdu610064China!NationalandLocalJointEngineeli曙Laborator)‘()/EnergyPlantsBiologicalF~telOilPtepara
4、tionandUlilizalion,Chengdu610225,China蚴ThisstudyaimedtooptimizethedegradationrateofphorbolesterofJarophacurcasseedcakethroughsolid—statefermentationofKlebsiellavariicola.Firstly,bythesinglefactorexperiments,theeffectsoffermentationtemperature,inoculumamount,initialamountofwaterandfermentationtime
5、weredetermined.ThenDesignExpert8.0wasusedtoanalyzeandoptimizethemodelof10horbolestersdegradationratebythreefactors(fermentationtemperature,inoculumamountandinitialamountofwater)throughresponsesurfacemethodology.Amodelwasthensetup,itsvalidityverified,andthedegradationrateofcurcinmeasured.Themodelw
6、assignificant(尸<0.001),fittingwel1.Inconfirmingexperiments,undertheconditionoffermentationtemperature37。C,inoculation(V/m)33%,theinitialwatercontent105%(V/m),afterfermentationfor48hours.thephorbolesterdegradationratewas84.30%andcurcindegradationrate63.21%.Theresultssuggestedthatwithoptimizationof
7、theresponsesurfacemethodology,variicolacandegradephorbolestereficientlyandatthesametimedegradecurcin.盈Jatrophacurcasseedcake;phorbolesters;Klebsiellavariicola,。solid—statefermentation;curcin麻疯树(JatrophacurcasL.)又名膏桐、小桐
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